Metabotropic Glutamate Receptor 1 ELISA Kit

Catalog No. ABIN5654674

Product Details Metabotropic Glutamate Receptor 1 ELISA Kit

Target: Metabotropic Glutamate Receptor 1 (GRM1) (Glutamate Receptor, Metabotropic 1 (GRM1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Glutamate Receptor, Metabotropic 1 (GRM1). No significant cross-reactivity or interference between Glutamate Receptor, Metabotropic 1 (GRM1) and analogues was observed.

Sensitivity: 0.058 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamate Receptor, Metabotropic 1 (GRM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutamate Receptor, Metabotropic 1 (GRM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutamate Receptor, Metabotropic 1 (GRM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutamate Receptor, Metabotropic 1 (GRM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutamate Receptor, Metabotropic 1 (GRM1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutamate Receptor, Metabotropic 1 (GRM1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Metabotropic Glutamate Receptor 1

Target: Metabotropic Glutamate Receptor 1 (GRM1) (Glutamate Receptor, Metabotropic 1 (GRM1))

Alternative Name: Glutamate Receptor, Metabotropic 1 (GRM1 Products)

Synonyms: fj39d05 ELISA Kit, wu:fj39d05 ELISA Kit, si:ch211-51a19.1 ELISA Kit, Glura ELISA Kit, mGlutR ELISA Kit, GB18621 ELISA Kit, GPRC1A ELISA Kit, MGLU1 ELISA Kit, MGLUR1 ELISA Kit, SCAR13 ELISA Kit, 4930455H15Rik ELISA Kit, ENSMUSG00000075319 ELISA Kit, Gm10828 ELISA Kit, Gprc1a ELISA Kit, mGluR1 ELISA Kit, nmf373 ELISA Kit, rcw ELISA Kit, wobl ELISA Kit, glutamate metabotropic receptor 1 ELISA Kit, glutamate receptor, metabotropic 1a ELISA Kit, metabotropic glutamate receptor 1 ELISA Kit, glutamate receptor, metabotropic 1 ELISA Kit, GRM1 ELISA Kit, grm1a ELISA Kit, mGlutR1 ELISA Kit, Grm1 ELISA Kit

Background:

Gene Name: Glutamate Receptor, Metabotropic 1

Gene Aliases: GPRC1A, GRM1A, MGLUR1, MGLUR1A, mGluR1

Gene ID: 2911

UniProt: Q13255