Glutamic Acid Decarboxylase ELISA Kit

Catalog No. ABIN5654675

Human Glutamic Acid Decarboxylase ELISA Kit, Colorimetric assay for quantification of Human Glutamic Acid Decarboxylase.

Quick Overview for Glutamic Acid Decarboxylase ELISA Kit (ABIN5654675)

Target: Glutamic Acid Decarboxylase (GAD)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details Glutamic Acid Decarboxylase ELISA Kit

Minimum Detection Limit: 1.56 ng/mL

Purpose: Human Glutamic Acid Decarboxylase (Anti-GAD) Anti-antigen ELISA Kit for detecting Anti-GAD in serum, plasma

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Glutamic Acid Decarboxylase (GAD). No significant cross-reactivity or interference between Glutamic Acid Decarboxylase (GAD) and analogues was observed.

Sensitivity: 0.59 ng/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Sample Volume: 100 μL

Assay Time: 2.5 h

Plate: Pre-coated

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutamic Acid Decarboxylase (GAD) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutamic Acid Decarboxylase (GAD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 12 months

Target Details for Glutamic Acid Decarboxylase

Target: Glutamic Acid Decarboxylase (GAD)

Alternative Name: Glutamic Acid Decarboxylase

Background: Gene Aliases: Glutamate Decarboxylase

Gene ID: 2571

UniProt: Q99259