Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

GP1BB ELISA Kit (Glycoprotein Ib (Platelet), beta Polypeptide) ELISA Kit

GP1BB Reactivity: Mouse Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma
Catalog No. ABIN5654765
$951.54
Plus shipping costs $45.00
96 tests
local_shipping Shipping to: United States
Delivery in 8 to 12 Business Days
  • Target
    Reactivity
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    31.25 pg/mL - 2000 pg/mL
    Minimum Detection Limit
    31.25 pg/mL
    Application
    ELISA
    Sample Type
    Plasma
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Glycoprotein Ib Beta Polypeptide, Platelet (GP1Bb). No significant cross-reactivity or interference between Glycoprotein Ib Beta Polypeptide, Platelet (GP1Bb) and analogues was observed.
    Sensitivity
    14.7 pg/mL
  • Target
    Alternative Name
    Glycoprotein Ib Beta Polypeptide, Platelet (GP1BB ELISA Kit Abstract)
    Background

    Gene Name: Glycoprotein Ib Beta Polypeptide, Platelet

    Gene Aliases: CD42c,CD42-c, GP1-BB, GPIB-B

    Gene ID
    14724
    UniProt
    P56400
  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycoprotein Ib Beta Polypeptide, Platelet (GP1Bb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glycoprotein Ib Beta Polypeptide, Platelet (GP1Bb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycoprotein Ib Beta Polypeptide, Platelet (GP1Bb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glycoprotein Ib Beta Polypeptide, Platelet (GP1Bb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glycoprotein Ib Beta Polypeptide, Platelet (GP1Bb) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glycoprotein Ib Beta Polypeptide, Platelet (GP1Bb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
You are here:
help Support