GNRH1 ELISA Kit

Catalog No. ABIN5654793

Product Details GNRH1 ELISA Kit

Target: GNRH1 (Gonadotropin-Releasing Hormone 1 (Luteinizing-Releasing Hormone) (GNRH1))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 12.35 pg/mL - 1000 pg/mL

Minimum Detection Limit: 12.35 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Gonadotropin Releasing Hormone (GnRH). No significant cross-reactivity or interference between Gonadotropin Releasing Hormone (GnRH) and analogues was observed.

Sensitivity: 5.25 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 h

Plate: Pre-coated

Protocol: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Gonadotropin Releasing Hormone (GnRH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Gonadotropin Releasing Hormone (GnRH) and unlabeled Gonadotropin Releasing Hormone (GnRH) (Standards or samples) with the pre-coated antibody specific to Gonadotropin Releasing Hormone (GnRH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Gonadotropin Releasing Hormone (GnRH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Gonadotropin Releasing Hormone (GnRH) in the sample.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Gonadotropin Releasing Hormone (GnRH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Gonadotropin Releasing Hormone (GnRH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for GNRH1

Target: GNRH1 (Gonadotropin-Releasing Hormone 1 (Luteinizing-Releasing Hormone) (GNRH1))

Alternative Name: Gonadotropin Releasing Hormone (GNRH1 Products)

Synonyms: GNRH ELISA Kit, GRH ELISA Kit, HH12 ELISA Kit, LHRH ELISA Kit, LNRH ELISA Kit, Gnrh ELISA Kit, Gnrh2 ELISA Kit, Lhrh1 ELISA Kit, Lnrh ELISA Kit, hpg ELISA Kit, Gnrha ELISA Kit, Lhrh ELISA Kit, Rgnrhg1 ELISA Kit, CGNRH-I ELISA Kit, GnRH-I ELISA Kit, GnRH ELISA Kit, gnrh ELISA Kit, gnrh1 ELISA Kit, grh ELISA Kit, lhrh ELISA Kit, lnrh ELISA Kit, mdGnRH ELISA Kit, mdgnrh ELISA Kit, mfGnRH ELISA Kit, gonadotropin releasing hormone 1 ELISA Kit, gonadotropin releasing hormone 1 L homeolog ELISA Kit, prepro-gonadotropin-releasing hormone ELISA Kit, GNRH1 ELISA Kit, Gnrh1 ELISA Kit, gnrh1.L ELISA Kit, gnrh1 ELISA Kit

Background:

Gene Name: Gonadotropin Releasing Hormone

Gene Aliases: GNRH1, GRH, LNRH, LHRH, LH-RH I, Luliberin, Luteinizing-Hormone Releasing Hormone, Progonadoliberin-1, Gonadoliberin I, Gonadorelin, GnRH-associated peptide 1

Gene ID: 25194

UniProt: P07490

Pathways: Hormone Activity