GDA ELISA Kit

Catalog No. ABIN5654920

Product Details GDA ELISA Kit

Target: GDA (Guanine Deaminase (GDA))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Guanine Deaminase (GDA). No significant cross-reactivity or interference between Guanine Deaminase (GDA) and analogues was observed.

Sensitivity: 0.129 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Guanine Deaminase (GDA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Guanine Deaminase (GDA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Guanine Deaminase (GDA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Guanine Deaminase (GDA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Guanine Deaminase (GDA) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Guanine Deaminase (GDA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for GDA

Target: GDA (Guanine Deaminase (GDA))

Alternative Name: Guanine Deaminase (GDA Products)

Synonyms: CYPIN ELISA Kit, GUANASE ELISA Kit, NEDASIN ELISA Kit, AU015411 ELISA Kit, AW047581 ELISA Kit, Cypin ELISA Kit, MGC81835 ELISA Kit, zgc:112282 ELISA Kit, AGR_C_4212 ELISA Kit, DDBDRAFT_0169373 ELISA Kit, DDBDRAFT_0230211 ELISA Kit, DDB_0169373 ELISA Kit, DDB_0230211 ELISA Kit, guanine deaminase ELISA Kit, guanine deaminase L homeolog ELISA Kit, short-chain dehydrogenase/reductase sdr ELISA Kit, GDA ELISA Kit, Gda ELISA Kit, gda.L ELISA Kit, gda ELISA Kit, SPO2956 ELISA Kit, Gdia_3192 ELISA Kit, Mchl_2046 ELISA Kit, Mnod_4655 ELISA Kit, guaD ELISA Kit, Dd586_1771 ELISA Kit, Kvar_2512 ELISA Kit, Mrub_1462 ELISA Kit, BC1002_2263 ELISA Kit, Cseg_1079 ELISA Kit, Mesil_1795 ELISA Kit, Trad_2850 ELISA Kit, Mesop_0346 ELISA Kit, gDA ELISA Kit, ML5_2802 ELISA Kit

Background:

Gene Name: Guanine Deaminase

Gene Aliases: CYPIN, Guanase, Guanine aminase, Guanine aminohydrolase, p51-nedasin

Gene ID: 9615

UniProt: Q9Y2T3