HSPD1 ELISA Kit

Catalog No. ABIN5654954

Product Details HSPD1 ELISA Kit

Target: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.234 ng/mL - 15 ng/mL

Minimum Detection Limit: 0.234 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Heat Shock 60kD Protein 1, Chaperonin (HSPD1). No significant cross-reactivity or interference between Heat Shock 60kD Protein 1, Chaperonin (HSPD1) and analogues was observed.

Sensitivity: 0.11 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock 60kD Protein 1, Chaperonin (HSPD1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock 60kD Protein 1, Chaperonin (HSPD1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock 60kD Protein 1, Chaperonin (HSPD1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock 60kD Protein 1, Chaperonin (HSPD1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heat Shock 60kD Protein 1, Chaperonin (HSPD1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heat Shock 60kD Protein 1, Chaperonin (HSPD1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for HSPD1

Target: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Alternative Name: Heat Shock 60kD Protein 1, Chaperonin (HSPD1 Products)

Synonyms: CPN60 ELISA Kit, GROEL ELISA Kit, HLD4 ELISA Kit, HSP-60 ELISA Kit, HSP60 ELISA Kit, HSP65 ELISA Kit, HuCHA60 ELISA Kit, SPG13 ELISA Kit, chaperonin ELISA Kit, cpn60 ELISA Kit, groel ELISA Kit, hld4 ELISA Kit, hsp60 ELISA Kit, hsp65 ELISA Kit, spg13 ELISA Kit, 60kDa ELISA Kit, Hsp60 ELISA Kit, 12 ELISA Kit, BP5 ELISA Kit, CG12101 ELISA Kit, Cpn60 ELISA Kit, Dmel\\CG12101 ELISA Kit, Dmhsp60 ELISA Kit, G62 ELISA Kit, HSP60A ELISA Kit, Hsp60A ELISA Kit, IEF16 ELISA Kit, Mmp-P1 ELISA Kit, SSP 7506 ELISA Kit, d-hsp60 ELISA Kit, hsp60A ELISA Kit, l(1)10Ac ELISA Kit, l(1)BP5 ELISA Kit, l(1)G8 ELISA Kit, l(1)HM21 ELISA Kit, l(1)L12 ELISA Kit, l(1)dp025 ELISA Kit, cb863 ELISA Kit, fa04a05 ELISA Kit, fb22d10 ELISA Kit, fi27b05 ELISA Kit, id:ibd2197 ELISA Kit, sb:cb144 ELISA Kit, wu:fa04a05 ELISA Kit, wu:fb22d10 ELISA Kit, wu:fi04a12 ELISA Kit, wu:fi27b05 ELISA Kit, MIF4 ELISA Kit, MNA2 ELISA Kit, mopA ELISA Kit, groL ELISA Kit, crpA ELISA Kit, Hspd1-30p ELISA Kit, heat shock protein family D (Hsp60) member 1 ELISA Kit, heat shock protein family D (Hsp60) member 1 S homeolog ELISA Kit, 60 kDa heat shock protein, mitochondrial ELISA Kit, heat shock protein 1 (chaperonin) ELISA Kit, Heat shock protein 60A ELISA Kit, heat shock 60 protein 1 ELISA Kit, chaperone ATPase HSP60 ELISA Kit, molecular chaperone GroEL ELISA Kit, thermosome subunit ELISA Kit, chaperonin GroEL ELISA Kit, mitochondrial chaperonin ELISA Kit, heat shock protein family D member 1 ELISA Kit, HSPD1 ELISA Kit, hspd1.S ELISA Kit, hspd1 ELISA Kit, LOC100414401 ELISA Kit, Hspd1 ELISA Kit, Hsp60A ELISA Kit, HSP60 ELISA Kit, groEL ELISA Kit, MMP_RS07785 ELISA Kit, groEl ELISA Kit, LOC100136430 ELISA Kit

Background:

Gene Name: Heat Shock 60kD Protein 1, Chaperonin

Gene Aliases: HSP60, GROEL, CPN60, HuCHA60, Heat Shock Protein 60, Spastic Paraplegia 13,Autosomal Dominant, 60 kDa chaperonin, Mitochondrial matrix protein P1, P60 lymphocyte protein

Gene ID: 15510

UniProt: P63038

Pathways: Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity