Hsc70 ELISA Kit

Catalog No. ABIN5654970

Product Details Hsc70 ELISA Kit

Target: Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Heat Shock 70 kDa Protein 8 (HSPA8). No significant cross-reactivity or interference between Heat Shock 70 kDa Protein 8 (HSPA8) and analogues was observed.

Sensitivity: 0.116 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock 70 kDa Protein 8 (HSPA8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock 70 kDa Protein 8 (HSPA8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock 70 kDa Protein 8 (HSPA8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock 70 kDa Protein 8 (HSPA8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heat Shock 70kDa Protein 8 (HSPA8) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heat Shock 70kDa Protein 8 (HSPA8) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Hsc70

Target: Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

Alternative Name: Heat Shock 70kDa Protein 8 (HSPA8 Products)

Synonyms: hsc54 ELISA Kit, hsc70 ELISA Kit, hsc71 ELISA Kit, hsp71 ELISA Kit, hsp73 ELISA Kit, hspa10 ELISA Kit, lap1 ELISA Kit, nip71 ELISA Kit, HSC54 ELISA Kit, HSC70 ELISA Kit, HSC71 ELISA Kit, HSP71 ELISA Kit, HSP73 ELISA Kit, HSPA10 ELISA Kit, LAP1 ELISA Kit, NIP71 ELISA Kit, Hsc70 ELISA Kit, 2410008N15Rik ELISA Kit, Hsc71 ELISA Kit, Hsc73 ELISA Kit, Hsp73 ELISA Kit, Hspa10 ELISA Kit, wu:fb01g06 ELISA Kit, wu:fi48b06 ELISA Kit, heat shock protein family A (Hsp70) member 8 L homeolog ELISA Kit, heat shock protein family A (Hsp70) member 8 ELISA Kit, heat shock 70kDa protein 8 ELISA Kit, heat shock protein 8 ELISA Kit, hspa8.L ELISA Kit, HSPA8 ELISA Kit, Hspa8 ELISA Kit, hspa8 ELISA Kit

Background:

Gene Name: Heat Shock 70 kDa Protein 8

Gene Aliases: LAP1, HSC54, HSC70, HSC71, HSP71, HSPA10, NIP71, HSP73, Heat shock cognate 71 kDa protein, Lipopolysaccharide-associated protein 1, LPS-associated protein 1

Gene ID: 15481

UniProt: P63017