HMOX2 ELISA Kit

Catalog No. ABIN5655014

Product Details HMOX2 ELISA Kit

Target: HMOX2 (Heme Oxygenase (Decycling) 2 (HMOX2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Heme Oxygenase 2, Decycling (HO2). No significant cross-reactivity or interference between Heme Oxygenase 2, Decycling (HO2) and analogues was observed.

Sensitivity: 0.059 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heme Oxygenase 2, Decycling (HO2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heme Oxygenase 2, Decycling (HO2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heme Oxygenase 2, Decycling (HO2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heme Oxygenase 2, Decycling (HO2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heme Oxygenase 2, Decycling (HO2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heme Oxygenase 2, Decycling (HO2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for HMOX2

Target: HMOX2 (Heme Oxygenase (Decycling) 2 (HMOX2))

Alternative Name: Heme Oxygenase 2, Decycling (HMOX2 Products)

Synonyms: HO-2 ELISA Kit, HO2 ELISA Kit, HMOX2 ELISA Kit, Ho-2 ELISA Kit, im:6909100 ELISA Kit, si:dkey-44g23.7 ELISA Kit, AtHO2 ELISA Kit, T9J22.22 ELISA Kit, T9J22_22 ELISA Kit, heme oxygenase 2 ELISA Kit, hmox2 ELISA Kit, ho-2 ELISA Kit, heme oxygenase 2 ELISA Kit, Heme oxygenase 2 ELISA Kit, heme oxygenase 2, putative ELISA Kit, heme oxygenase 2a ELISA Kit, heme oxygenase 2 L homeolog ELISA Kit, HMOX2 ELISA Kit, Hmox2 ELISA Kit, HO2 ELISA Kit, ho2 ELISA Kit, CNF01200 ELISA Kit, CPR_0203 ELISA Kit, CGB_F5120W ELISA Kit, hmox2a ELISA Kit, hmox2.L ELISA Kit

Background:

Gene Name: Heme Oxygenase 2, Decycling

Gene Aliases: HMOX2

Gene ID: 3163

UniProt: P30519

Pathways: Transition Metal Ion Homeostasis