HNF1B ELISA Kit

Catalog No. ABIN5655092

Product Details HNF1B ELISA Kit

Target: HNF1B (HNF1 Homeobox B (HNF1B))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Hepatocyte Nuclear Factor 1 Beta (HNF1b). No significant cross-reactivity or interference between Hepatocyte Nuclear Factor 1 Beta (HNF1b) and analogues was observed.

Sensitivity: 0.058 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hepatocyte Nuclear Factor 1 Beta (HNF1b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hepatocyte Nuclear Factor 1 Beta (HNF1b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hepatocyte Nuclear Factor 1 Beta (HNF1b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hepatocyte Nuclear Factor 1 Beta (HNF1b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hepatocyte Nuclear Factor 1 Beta (HNF1b) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hepatocyte Nuclear Factor 1 Beta (HNF1b) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for HNF1B

Target: HNF1B (HNF1 Homeobox B (HNF1B))

Alternative Name: Hepatocyte Nuclear Factor 1 Beta (HNF1B Products)

Synonyms: FJHN ELISA Kit, HNF-1B ELISA Kit, HNF1beta ELISA Kit, HNF2 ELISA Kit, HPC11 ELISA Kit, LF-B3 ELISA Kit, LFB3 ELISA Kit, MODY5 ELISA Kit, TCF-2 ELISA Kit, TCF2 ELISA Kit, VHNF1 ELISA Kit, HNF-1b ELISA Kit, HNF1B ELISA Kit, AI385728 ELISA Kit, AI987804 ELISA Kit, HNF-1Beta ELISA Kit, Hnf1beta ELISA Kit, Tcf-2 ELISA Kit, Tcf2 ELISA Kit, vHNF1 ELISA Kit, vHNF-1 ELISA Kit, chunp6877 ELISA Kit, hnf1b ELISA Kit, mst ELISA Kit, tcf2 ELISA Kit, vhnf1 ELISA Kit, lfb3 ELISA Kit, hnf-1beta ELISA Kit, hnf1-beta ELISA Kit, hnf1beta ELISA Kit, HNF1g ELISA Kit, wu:fc23f06 ELISA Kit, HNF1 homeobox B ELISA Kit, HNF1 homeobox Ba ELISA Kit, HNF1 homeobox B S homeolog ELISA Kit, HNF1 homeobox B L homeolog ELISA Kit, HNF1 homeobox Bb ELISA Kit, HNF1B ELISA Kit, Hnf1b ELISA Kit, hnf1ba ELISA Kit, hnf1b.S ELISA Kit, hnf1b.L ELISA Kit, hnf1bb ELISA Kit

Background:

Gene Name: Hepatocyte Nuclear Factor 1 Beta

Gene Aliases: FJHN, TCF2, HNF1beta, HNF2, LFB3, LF-B3, MODY5, VHNF1, Transcription Factor 2,Hepatic, Variant Hepatic Nuclear Factor, Homeoprotein LFB3

Pathways: Hormone Transport, Stem Cell Maintenance, Tube Formation