HDGF ELISA Kit

Catalog No. ABIN5655094

Product Details HDGF ELISA Kit

Target: HDGF (Hepatoma-Derived Growth Factor (HDGF))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.2 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Hepatoma Derived Growth Factor (HDGF). No significant cross-reactivity or interference between Hepatoma Derived Growth Factor (HDGF) and analogues was observed.

Sensitivity: 12.4 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hepatoma Derived Growth Factor (HDGF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hepatoma Derived Growth Factor (HDGF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hepatoma Derived Growth Factor (HDGF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hepatoma Derived Growth Factor (HDGF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hepatoma Derived Growth Factor (HDGF) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hepatoma Derived Growth Factor (HDGF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for HDGF

Target: HDGF (Hepatoma-Derived Growth Factor (HDGF))

Alternative Name: Hepatoma Derived Growth Factor (HDGF Products)

Synonyms: HMG1L2 ELISA Kit, hmg1l2 ELISA Kit, HDGF ELISA Kit, DKFZp459H185 ELISA Kit, AI118077 ELISA Kit, D3Ertd299e ELISA Kit, heparin binding growth factor ELISA Kit, hepatoma-derived growth factor ELISA Kit, hepatoma-derived growth factor (high-mobility group protein 1-like) ELISA Kit, heparin binding growth factor L homeolog ELISA Kit, hepatoma-derived growth factor-like ELISA Kit, HDGF ELISA Kit, hdgf ELISA Kit, Hdgf ELISA Kit, hdgf.L ELISA Kit, LOC100357981 ELISA Kit

Background:

Gene Name: Hepatoma Derived Growth Factor

Gene Aliases: HMG1L2, High-Mobility Group Protein 1-Like 2

Gene ID: 15191

UniProt: P51859

Pathways: ER-Nucleus Signaling