Hepcidin CLIA Kit

Catalog No. ABIN5655100

Product Details Hepcidin CLIA Kit

Target: Hepcidin (HAMP) (Hepcidin Antimicrobial Peptide (HAMP))

Reactivity: Human

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Minimum Detection Limit: 62.5 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Hepcidin (Hepc). No significant cross-reactivity or interference between Hepcidin (Hepc) and analogues was observed.

Sensitivity: 2.35 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Hepcidin (Hepc). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Hepcidin (Hepc). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Hepcidin (Hepc) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hepcidin (Hepc) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hepcidin (Hepc) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: Hepcidin (HAMP) (Hepcidin Antimicrobial Peptide (HAMP))

Alternative Name: Hepcidin (HAMP Products)

Synonyms: HEPC CLIA Kit, HFE2B CLIA Kit, LEAP1 CLIA Kit, PLTR CLIA Kit, Hepcidin CLIA Kit, Hamp1 CLIA Kit, Hepc CLIA Kit, Hepc1 CLIA Kit, HAMP CLIA Kit, hepcidin CLIA Kit, hamp1 CLIA Kit, hep1 CLIA Kit, hepcidin antimicrobial peptide CLIA Kit, hepcidin-like CLIA Kit, hepcidin-1 CLIA Kit, Ham percentage CLIA Kit, HAMP CLIA Kit, Hamp CLIA Kit, hamp CLIA Kit, LOC100135935 CLIA Kit, LOC106584587 CLIA Kit

Background:

Gene Name: Hepcidin

Gene Aliases: HAMP, HFE2B, PLTR, LEAP1, Hepcidin Antimicrobial Peptide, Liver-expressed antimicrobial peptide 1, Putative liver tumor regressor

Gene ID: 57817

UniProt: P81172

Pathways: Hormone Activity, Transition Metal Ion Homeostasis