HDAC1 ELISA Kit

Catalog No. ABIN5655167

Product Details HDAC1 ELISA Kit

Target: HDAC1 (Histone Deacetylase 1 (HDAC1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.781 ng/mL - 50 ng/mL

Minimum Detection Limit: 0.781 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Histone Deacetylase 1 (HDAC1). No significant cross-reactivity or interference between Histone Deacetylase 1 (HDAC1) and analogues was observed.

Sensitivity: 0.27 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histone Deacetylase 1 (HDAC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histone Deacetylase 1 (HDAC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histone Deacetylase 1 (HDAC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Histone Deacetylase 1 (HDAC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Histone Deacetylase 1 (HDAC1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Histone Deacetylase 1 (HDAC1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for HDAC1

Target: HDAC1 (Histone Deacetylase 1 (HDAC1))

Alternative Name: Histone Deacetylase 1 (HDAC1 Products)

Synonyms: GON-10 ELISA Kit, HD1 ELISA Kit, RPD3 ELISA Kit, RPD3L1 ELISA Kit, CG7471 ELISA Kit, DHDAC1 ELISA Kit, DRpd3 ELISA Kit, DmHDAC1 ELISA Kit, Dmel\\CG7471 ELISA Kit, E(var)3-64BC ELISA Kit, HDAC ELISA Kit, HDAC-1 ELISA Kit, HDAC1 ELISA Kit, Hdac1 ELISA Kit, Rpd3/HDAC ELISA Kit, Su(var)3-26 ELISA Kit, Su(var)326 ELISA Kit, Su(var)328 ELISA Kit, dHDAC-1 ELISA Kit, dHDAC1 ELISA Kit, dRPD3 ELISA Kit, dRpd3 ELISA Kit, dmHDA401 ELISA Kit, drpd3 ELISA Kit, hdac1 ELISA Kit, l(3)04556 ELISA Kit, l(3)64Cc ELISA Kit, rpd3 ELISA Kit, rpd[3] ELISA Kit, gon-10 ELISA Kit, hdac1b ELISA Kit, rpd3l1 ELISA Kit, Rpd3 ELISA Kit, GB14706 ELISA Kit, hdac1-b ELISA Kit, chunp6919 ELISA Kit, hdac-1 ELISA Kit, mp:zf637-2-001987 ELISA Kit, wu:fb19h11 ELISA Kit, wu:fi06f03 ELISA Kit, zgc:101582 ELISA Kit, zgc:65818 ELISA Kit, Hdac1-ps ELISA Kit, MommeD5 ELISA Kit, ARABIDOPSIS HISTONE DEACETYLASE 1 ELISA Kit, ARABIDOPSIS HISTONE DEACETYLASE 19 ELISA Kit, ATHD1 ELISA Kit, ATHDA19 ELISA Kit, ATRPD3A ELISA Kit, F20D10.250 ELISA Kit, F20D10_250 ELISA Kit, HDA1 ELISA Kit, HDA19 ELISA Kit, HISTONE DEACETYLASE ELISA Kit, HISTONE DEACETYLASE 19 ELISA Kit, HISTONE DEACETYLASE19 ELISA Kit, RPD3A ELISA Kit, histone deacetylase 1 ELISA Kit, HDM ELISA Kit, ab21 ELISA Kit, hdac1a ELISA Kit, histone deacetylase 1 ELISA Kit, Histone deacetylase 1 ELISA Kit, histone deacetylase ELISA Kit, histone deacetylase 1 S homeolog ELISA Kit, histone deacetylase Rpd3 ELISA Kit, histone deacetylase 1 L homeolog ELISA Kit, HDAC1 ELISA Kit, hdac1.S ELISA Kit, LOC411503 ELISA Kit, hdac1 ELISA Kit, Hdac1 ELISA Kit, HD1 ELISA Kit, hda-1 ELISA Kit, hdac1.L ELISA Kit, LOC748850 ELISA Kit

Background:

Gene Name: Histone Deacetylase 1

Gene Aliases: HDAC1, GON10, HD1, RPD3, RPD3L1

Pathways: Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Mitotic G1-G1/S Phases, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Negative Regulation of intrinsic apoptotic Signaling, Embryonic Body Morphogenesis