HDAC9 ELISA Kit

Catalog No. ABIN5655177

Product Details HDAC9 ELISA Kit

Target: HDAC9 (Histone Deacetylase 9 (HDAC9))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Histone Deacetylase 9 (HDAC9). No significant cross-reactivity or interference between Histone Deacetylase 9 (HDAC9) and analogues was observed.

Sensitivity: 0.138 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histone Deacetylase 9 (HDAC9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histone Deacetylase 9 (HDAC9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histone Deacetylase 9 (HDAC9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Histone Deacetylase 9 (HDAC9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Histone Deacetylase 9 (HDAC9) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Histone Deacetylase 9 (HDAC9) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for HDAC9

Target: HDAC9 (Histone Deacetylase 9 (HDAC9))

Alternative Name: Histone Deacetylase 9 (HDAC9 Products)

Synonyms: HD7 ELISA Kit, HD7b ELISA Kit, HD9 ELISA Kit, HDAC ELISA Kit, HDAC7 ELISA Kit, HDAC7B ELISA Kit, HDAC9B ELISA Kit, HDAC9FL ELISA Kit, HDRP ELISA Kit, MITR ELISA Kit, AV022454 ELISA Kit, D030072B18Rik ELISA Kit, HD7B ELISA Kit, Hdac7b ELISA Kit, Mitr ELISA Kit, mKIAA0744 ELISA Kit, hdac9 ELISA Kit, zgc:73392 ELISA Kit, TWIST ELISA Kit, HDAC9 ELISA Kit, DKFZp459E171 ELISA Kit, HDA09 ELISA Kit, HISTONE DEACETYLASE ELISA Kit, histone deacetylase 9 ELISA Kit, RGD1310748 ELISA Kit, RGD1563092 ELISA Kit, hdac9-A ELISA Kit, histone deacetylase 9 ELISA Kit, histone deacetylase 9b ELISA Kit, histone deacetylase 9 S homeolog ELISA Kit, HDAC9 ELISA Kit, Hdac9 ELISA Kit, hdac9b ELISA Kit, HDA9 ELISA Kit, hdac9.S ELISA Kit

Background:

Gene Name: Histone Deacetylase 9

Gene Aliases: HD7, HDAC, HDAC7, HDAC7B, HDAC9B, HDAC9FL, HDRP, MITR, Histone deacetylase-related protein, MEF2-interacting transcription repressor MITR, Histone deacetylase 7B

Gene ID: 9734

UniProt: Q9UKV0

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development