IGFBP7 CLIA Kit
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- Target See all IGFBP7 CLIA Kits
- IGFBP7 (Insulin-Like Growth Factor Binding Protein 7 (IGFBP7))
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Reactivity
- Human
- Detection Method
- Chemiluminescent
- Method Type
- Sandwich ELISA
- Detection Range
- 9.38 ng/mL - 600 ng/mL
- Minimum Detection Limit
- 9.38 ng/mL
- Application
- ELISA
- Sample Type
- Plasma, Serum
- Analytical Method
- Quantitative
- Specificity
- This assay has high sensitivity and excellent specificity for detection of Insulin Like Growth Factor Binding Protein 7 (IGFBP7). No significant cross-reactivity or interference between Insulin Like Growth Factor Binding Protein 7 (IGFBP7) and analogues was observed.
- Sensitivity
- 0.31 ng/mL
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- Comment
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Assay Time
- 2 - 3 h
- Plate
- Pre-coated
- Protocol
- The microplate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor Binding Protein 7 (IGFBP7). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Insulin Like Growth Factor Binding Protein 7 (IGFBP7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Insulin Like Growth Factor Binding Protein 7 (IGFBP7) level in the sample or standard.,
- Assay Precision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Like Growth Factor Binding Protein 7 (IGFBP7) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Like Growth Factor Binding Protein 7 (IGFBP7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Handling Advice
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Storage
- 4 °C,-20 °C
- Storage Comment
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Expiry Date
- 4-8 months
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- Target See all IGFBP7 CLIA Kits
- IGFBP7 (Insulin-Like Growth Factor Binding Protein 7 (IGFBP7))
- Alternative Name
- Insulin Like Growth Factor Binding Protein 7 (IGFBP7 Products)
- Synonyms
- zgc:85888 CLIA Kit, AGM CLIA Kit, FSTL2 CLIA Kit, IBP-7 CLIA Kit, IGFBP-7 CLIA Kit, IGFBP-7v CLIA Kit, IGFBPRP1 CLIA Kit, MAC25 CLIA Kit, PSF CLIA Kit, RAMSVPS CLIA Kit, TAF CLIA Kit, Fstl2 CLIA Kit, Mac25 CLIA Kit, insulin like growth factor binding protein 7 CLIA Kit, insulin-like growth factor binding protein 7 CLIA Kit, IGFBP7 CLIA Kit, igfbp7 CLIA Kit, Igfbp7 CLIA Kit
- Background
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Gene Name: Insulin Like Growth Factor Binding Protein 7
Gene Aliases: FSTL2, IGFBP-7v, MAC25, PSF, TAF, IGFBP-rP1, MAC25 protein, Prostacyclin-stimulating factor, PGI2-stimulating factor, Tumor-derived adhesion factor
- Pathways
- Growth Factor Binding
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