IL-17 ELISA Kit

Catalog No. ABIN5655799

The Chicken IL-17 ELISA Kit (ABIN5655799) is a Colorimetric ELISA Kit designed to quantify Chicken IL-17.

Quick Overview for IL-17 ELISA Kit (ABIN5655799)

Target: IL-17 (IL17) (Interleukin 17 (IL17))

Reactivity: Chicken

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 62.5 pg/mL - 1000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Product Details IL-17 ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Interleukin 17 (IL17). No significant cross-reactivity or interference between Interleukin 17 (IL17) and analogues was observed.

Sensitivity: 25.4 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 h

Plate: Pre-coated

Protocol: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Interleukin 17 (IL17) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Interleukin 17 (IL17) and unlabeled Interleukin 17 (IL17) (Standards or samples) with the pre-coated antibody specific to Interleukin 17 (IL17). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Interleukin 17 (IL17) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Interleukin 17 (IL17) in the sample.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 17 (IL17) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 17 (IL17) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for IL-17

Target: IL-17 (IL17) (Interleukin 17 (IL17))

Alternative Name: Interleukin 17

Background:

Gene Name: Interleukin 17

Gene Aliases: IL17A, CTLA8, IL-17A, Cytotoxic T-Lymphocyte-Associated Protein 8

Gene ID: 395111

UniProt: Q7T1P7