IL-24 CLIA Kit

Catalog No. ABIN5655919

The Rat IL-24 ELISA Kit (ABIN5655919) is a Chemiluminescent ELISA Kit designed to quantify Rat IL-24.

Quick Overview for IL-24 CLIA Kit (ABIN5655919)

Target: IL-24 (IL24) (Interleukin 24 (IL24))

Reactivity: Rat

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 1.37 pg/mL - 1000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Product Details IL-24 CLIA Kit

Minimum Detection Limit: 1.37 pg/mL

Purpose: Rat Interleukin 24 (IL24) CLIA Kit for detecting IL24 in serum, plasma, tissue homogenates, cell lysates, cell culture supernates

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Interleukin 24 (IL24). No significant cross-reactivity or interference between Interleukin 24 (IL24) and analogues was observed.

Sensitivity: 0.49 pg/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• Substrate A
• Substrate B
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Interleukin 24 (IL24). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interleukin 24 (IL24). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interleukin 24 (IL24) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 24 (IL24) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 24 (IL24) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL-24 (IL24) (Interleukin 24 (IL24))

Alternative Name: Interleukin 24

Background: Gene Aliases: C49A, FISP, IL10B, MDA7, Mob-5, ST16, Mda-7, Melanoma Differentiation Association Protein 7, Suppression Of Tumorigenicity 16

Gene ID: 170819

UniProt: Q9JI24

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Autophagy