KEAP1 ELISA Kit

Catalog No. ABIN5656175

Product Details KEAP1 ELISA Kit

Target: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Kelch Like ECH Associated Protein 1 (KEAP1). No significant cross-reactivity or interference between Kelch Like ECH Associated Protein 1 (KEAP1) and analogues was observed.

Sensitivity: 0.056 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kelch Like ECH Associated Protein 1 (KEAP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kelch Like ECH Associated Protein 1 (KEAP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kelch Like ECH Associated Protein 1 (KEAP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kelch Like ECH Associated Protein 1 (KEAP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kelch Like ECH Associated Protein 1 (KEAP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kelch Like ECH Associated Protein 1 (KEAP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for KEAP1

Target: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Alternative Name: Kelch Like ECH Associated Protein 1 (KEAP1 Products)

Synonyms: INrf2 ELISA Kit, KLHL19 ELISA Kit, INRF2 ELISA Kit, mKIAA0132 ELISA Kit, Inrf2 ELISA Kit, fb36f11 ELISA Kit, keap1 ELISA Kit, wu:fb36f11 ELISA Kit, KEAP1 ELISA Kit, klhl19 ELISA Kit, zgc:171654 ELISA Kit, kelch like ECH associated protein 1 ELISA Kit, kelch-like ECH-associated protein 1 ELISA Kit, Kelch-like ECH-associated protein 1 ELISA Kit, kelch-like ECH-associated protein 1a ELISA Kit, kelch like ECH associated protein 1 L homeolog ELISA Kit, kelch-like ECH-associated protein 1b ELISA Kit, KEAP1 ELISA Kit, Keap1 ELISA Kit, keap1a ELISA Kit, keap1 ELISA Kit, keap1.L ELISA Kit, keap1b ELISA Kit

Background:

Gene Name: Kelch Like ECH Associated Protein 1

Gene Aliases: INrf2, KLHL19, Cytosolic inhibitor of Nrf2, Kelch-like protein 19

Gene ID: 9817

UniProt: Q14145

Pathways: Maintenance of Protein Location