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Cytokeratin 18 ELISA Kit

KRT18 Reactivity: Rat Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Catalog No. ABIN5656189
  • Target See all Cytokeratin 18 (KRT18) ELISA Kits
    Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))
    Reactivity
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Rat
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.156 ng/mL - 10 ng/mL
    Minimum Detection Limit
    0.156 ng/mL
    Application
    ELISA
    Sample Type
    Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Keratin 18 (KRT18). No significant cross-reactivity or interference between Keratin 18 (KRT18) and analogues was observed.
    Sensitivity
    0.058 ng/mL
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  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 18 (KRT18). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Keratin 18 (KRT18). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratin 18 (KRT18), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Keratin 18 (KRT18) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Keratin 18 (KRT18) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Keratin 18 (KRT18) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all Cytokeratin 18 (KRT18) ELISA Kits
    Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))
    Alternative Name
    Keratin 18 (KRT18 Products)
    Synonyms
    CK18 ELISA Kit, K18 ELISA Kit, Krt1-18 ELISA Kit, CYK18 ELISA Kit, DreK18 ELISA Kit, cb83 ELISA Kit, dapk1 ELISA Kit, sb:cb83 ELISA Kit, wu:fa13f02 ELISA Kit, wu:fb36b04 ELISA Kit, krt18 ELISA Kit, MGC64569 ELISA Kit, MGC75922 ELISA Kit, KRT18 ELISA Kit, CK-18-A ELISA Kit, K18-A ELISA Kit, NC-11 ELISA Kit, cyk18 ELISA Kit, k18 ELISA Kit, krt18-a ELISA Kit, krt18-b ELISA Kit, krt18a ELISA Kit, xkendob ELISA Kit, keratin 18 ELISA Kit, keratin 18, type I L homeolog ELISA Kit, keratin 18, type I ELISA Kit, keratin, type I cytoskeletal 18 ELISA Kit, K18, simple type I keratin ELISA Kit, Keratin, type I cytoskeletal 18 ELISA Kit, keratin 18, type I S homeolog ELISA Kit, Krt18 ELISA Kit, KRT18 ELISA Kit, krt18 ELISA Kit, krt18.L ELISA Kit, LOC700569 ELISA Kit, LOC100136778 ELISA Kit, k1c18 ELISA Kit, LOC100008885 ELISA Kit, krt18.S ELISA Kit
    Background

    Gene Name: Keratin 18

    Gene Aliases: CK18, CYK18, K18, Cytokeratin 18, Cell proliferation-inducing gene 46 protein, Keratin, type I cytoskeletal 18

    Gene ID
    294853
    UniProt
    Q5BJY9
    Pathways
    Apoptosis, Caspase Cascade in Apoptosis
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