LAP ELISA Kit

Catalog No. ABIN5656380

Product Details LAP ELISA Kit

Target: LAP (Leucine Aminopeptidase (LAP))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.781 ng/mL - 50 ng/mL

Minimum Detection Limit: 0.781 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Leucine Aminopeptidase (LAP). No significant cross-reactivity or interference between Leucine Aminopeptidase (LAP) and analogues was observed.

Sensitivity: 0.37 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leucine Aminopeptidase (LAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Leucine Aminopeptidase (LAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Leucine Aminopeptidase (LAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Leucine Aminopeptidase (LAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Leucine Aminopeptidase (LAP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Leucine Aminopeptidase (LAP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for LAP

Target: LAP (Leucine Aminopeptidase (LAP))

Alternative Name: Leucine Aminopeptidase (LAP Products)

Synonyms: DDBDRAFT_0218188 ELISA Kit, DDBDRAFT_0231024 ELISA Kit, DDB_0218188 ELISA Kit, DDB_0231024 ELISA Kit, 18.m06372 ELISA Kit, CALM ELISA Kit, CLTH ELISA Kit, LAP ELISA Kit, Calm ELISA Kit, leucine aminopeptidase ELISA Kit, leucine aminopeptidase, putative ELISA Kit, phosphatidylinositol binding clathrin assembly protein ELISA Kit, PVX_118180 ELISA Kit, CpipJ_CPIJ002841 ELISA Kit, lap ELISA Kit, pepA ELISA Kit, NAEGRDRAFT_78195 ELISA Kit, TP04_0561 ELISA Kit, BBOV_II004450 ELISA Kit, PKH_126020 ELISA Kit, NAEGRDRAFT_82309 ELISA Kit, PICALM ELISA Kit, Picalm ELISA Kit

Background:

Gene Name: Leucine Aminopeptidase

Gene Aliases: LAPEP, LAP3, PEPS, Peptidase S, Leucyl Aminopeptidase, Cytosol aminopeptidase, Proline aminopeptidase, Prolyl aminopeptidase

Gene ID: 289668

UniProt: Q68FS4