LRP1 ELISA Kit

Catalog No. ABIN5656490

Product Details LRP1 ELISA Kit

Target: LRP1 (Low Density Lipoprotein Receptor-Related Protein 1 (LRP1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Minimum Detection Limit: 62.5 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Low Density Lipoprotein Receptor Related Protein 1 (LRP1). No significant cross-reactivity or interference between Low Density Lipoprotein Receptor Related Protein 1 (LRP1) and analogues was observed.

Sensitivity: 28.4 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Low Density Lipoprotein Receptor Related Protein 1 (LRP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Low Density Lipoprotein Receptor Related Protein 1 (LRP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Low Density Lipoprotein Receptor Related Protein 1 (LRP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Low Density Lipoprotein Receptor Related Protein 1 (LRP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Low Density Lipoprotein Receptor Related Protein 1 (LRP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Low Density Lipoprotein Receptor Related Protein 1 (LRP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for LRP1

Target: LRP1 (Low Density Lipoprotein Receptor-Related Protein 1 (LRP1))

Alternative Name: Low Density Lipoprotein Receptor Related Protein 1 (LRP1 Products)

Synonyms: A2MR ELISA Kit, APOER ELISA Kit, APR ELISA Kit, CD91 ELISA Kit, IGFBP3R ELISA Kit, LRP ELISA Kit, LRP1A ELISA Kit, TGFBR5 ELISA Kit, A2mr ELISA Kit, AI316852 ELISA Kit, Lrp ELISA Kit, LRP-1 ELISA Kit, LDL receptor related protein 1 ELISA Kit, prolow-density lipoprotein receptor-related protein 1 ELISA Kit, low density lipoprotein receptor-related protein 1 ELISA Kit, LRP1 ELISA Kit, Lrp1 ELISA Kit, LOC100414789 ELISA Kit

Background:

Gene Name: Low Density Lipoprotein Receptor Related Protein 1

Gene Aliases: CD91, LRP, A2MR, APOER, APR, TGFBR5, Alpha-2-Macroglobulin Receptor, Prolow-density lipoprotein receptor-related protein 1, Apolipoprotein E receptor

Gene ID: 4035

UniProt: Q07954

Pathways: Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling