LHB ELISA Kit

Catalog No. ABIN5656514

Product Details LHB ELISA Kit

Target: LHB (Luteinizing Hormone beta Polypeptide (LHB))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 246.91 pg/mL - 20000 pg/mL

Minimum Detection Limit: 246.91 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Luteinizing Hormone Beta Polypeptide (LHb). No significant cross-reactivity or interference between Luteinizing Hormone Beta Polypeptide (LHb) and analogues was observed.

Sensitivity: 89.2 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 h

Plate: Pre-coated

Protocol: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Luteinizing Hormone Beta Polypeptide (LHb) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Luteinizing Hormone Beta Polypeptide (LHb) and unlabeled Luteinizing Hormone Beta Polypeptide (LHb) (Standards or samples) with the pre-coated antibody specific to Luteinizing Hormone Beta Polypeptide (LHb). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Luteinizing Hormone Beta Polypeptide (LHb) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Luteinizing Hormone Beta Polypeptide (LHb) in the sample.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Luteinizing Hormone Beta Polypeptide (LHb) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Luteinizing Hormone Beta Polypeptide (LHb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for LHB

Target: LHB (Luteinizing Hormone beta Polypeptide (LHB))

Alternative Name: Luteinizing Hormone Beta Polypeptide (LHB Products)

Synonyms: CGB4 ELISA Kit, LSH-B ELISA Kit, hLHB ELISA Kit, LH ELISA Kit, LH-B ELISA Kit, LSH-beta ELISA Kit, cgb4 ELISA Kit, hlhb ELISA Kit, xlhb ELISA Kit, lhb-A ELISA Kit, lsh-b ELISA Kit, LH/CG-beta ELISA Kit, LHB ELISA Kit, LSH-B/CG-B ELISA Kit, Lutropin beta chain ELISA Kit, lhb1 ELISA Kit, lhbeta1 ELISA Kit, zgc:92312 ELISA Kit, lhb ELISA Kit, luteinizing hormone beta polypeptide ELISA Kit, luteinizing hormone beta ELISA Kit, lutropin subunit beta-like ELISA Kit, luteinizing hormone, beta polypeptide ELISA Kit, luteinizing hormone beta polypeptide L homeolog ELISA Kit, LHB ELISA Kit, Lhb ELISA Kit, lhb ELISA Kit, LOC106838274 ELISA Kit, lhb.L ELISA Kit

Background:

Gene Name: Luteinizing Hormone Beta Polypeptide

Gene Aliases: LH-B, CGB4, LSH-B, hLHB, Lutropin Subunit Beta, Interstitial Cell Stimulating Hormone,Beta Chain

Gene ID: 3972

UniProt: P01229

Pathways: Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity, C21-Steroid Hormone Metabolic Process