LOXL2 ELISA Kit

Catalog No. ABIN5656567

Product Details LOXL2 ELISA Kit

Target: LOXL2 (Lysyl Oxidase-Like 2 (LOXL2))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Lysyl Oxidase Like Protein 2 (LOXL2). No significant cross-reactivity or interference between Lysyl Oxidase Like Protein 2 (LOXL2) and analogues was observed.

Sensitivity: 0.057 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lysyl Oxidase Like Protein 2 (LOXL2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lysyl Oxidase Like Protein 2 (LOXL2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lysyl Oxidase Like Protein 2 (LOXL2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lysyl Oxidase Like Protein 2 (LOXL2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lysyl Oxidase Like Protein 2 (LOXL2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lysyl Oxidase Like Protein 2 (LOXL2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for LOXL2

Target: LOXL2 (Lysyl Oxidase-Like 2 (LOXL2))

Alternative Name: Lysyl Oxidase Like Protein 2 (LOXL2 Products)

Synonyms: LOR2 ELISA Kit, WS9-14 ELISA Kit, CG4402 ELISA Kit, Dmel\\CG4402 ELISA Kit, Dmloxl-2 ELISA Kit, dmlox-2 ELISA Kit, 1110004B06Rik ELISA Kit, 4930526G11Rik ELISA Kit, 9430067E15Rik ELISA Kit, GB13360 ELISA Kit, lox2-like ELISA Kit, LOXL2 ELISA Kit, lor2 ELISA Kit, loxl-2 ELISA Kit, ws9-14 ELISA Kit, im:7136137 ELISA Kit, si:dkeyp-32b1.1 ELISA Kit, wu:fk12g04 ELISA Kit, zgc:158414 ELISA Kit, lysyl oxidase like 2 ELISA Kit, lysyl oxidase-like 2 ELISA Kit, lysyl oxidase homolog 4 ELISA Kit, lysyl oxidase like 2 L homeolog ELISA Kit, lysyl oxidase-like 2b ELISA Kit, LOXL2 ELISA Kit, lox2 ELISA Kit, Loxl2 ELISA Kit, LOC408544 ELISA Kit, loxl2.L ELISA Kit, loxl2 ELISA Kit, loxl2b ELISA Kit

Background:

Gene Name: Lysyl Oxidase Like Protein 2

Gene Aliases: LOR2, WS9-14, Lysyl oxidase homolog 2, Lysyl oxidase-related protein 2, Lysyl oxidase-related protein WS9-14

Gene ID: 290350

UniProt: B5DF27

Pathways: Chromatin Binding