MBL2 ELISA Kit
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- Target See all MBL2 ELISA Kits
- MBL2 (Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2))
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 6.25 ng/mL - 400 ng/mL
- Minimum Detection Limit
- 6.25 ng/mL
- Application
- ELISA
- Sample Type
- Plasma, Serum
- Analytical Method
- Quantitative
- Specificity
- This assay has high sensitivity and excellent specificity for detection of Mannose Binding Lectin (MBL). No significant cross-reactivity or interference between Mannose Binding Lectin (MBL) and analogues was observed.
- Sensitivity
- 2.45 ng/mL
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- Comment
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Assay Time
- 2 - 3 h
- Plate
- Pre-coated
- Protocol
- This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to Mannose Binding Lectin (MBL) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between Horseradish Peroxidase (HRP) labeled Mannose Binding Lectin (MBL) and unlabeled Mannose Binding Lectin (MBL) (Standards or samples) with the pre-coated antibody specific to Mannose Binding Lectin (MBL). After incubation the unbound conjugate is washed off. The amount of bound HRP conjugate is reverse proportional to the concentration of Mannose Binding Lectin (MBL) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mannose Binding Lectin (MBL) in the sample.
- Assay Precision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mannose Binding Lectin (MBL) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mannose Binding Lectin (MBL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Handling Advice
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Storage
- 4 °C,-20 °C
- Storage Comment
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Expiry Date
- 4-8 months
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- Target See all MBL2 ELISA Kits
- MBL2 (Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2))
- Alternative Name
- Mannose Binding Lectin (MBL2 Products)
- Synonyms
- COLEC1 ELISA Kit, HSMBPC ELISA Kit, MBL ELISA Kit, MBL2D ELISA Kit, MBP ELISA Kit, MBP-C ELISA Kit, MBP1 ELISA Kit, MBPD ELISA Kit, pMBP-27 ELISA Kit, mbl2 ELISA Kit, MBL1 ELISA Kit, cMBl ELISA Kit, collectin ELISA Kit, Ab2-001 ELISA Kit, Ab2-011 ELISA Kit, L-MBP ELISA Kit, MBL-C ELISA Kit, COLEC2 ELISA Kit, mbl ELISA Kit, etID42583.2 ELISA Kit, fb68b07 ELISA Kit, hbl3 ELISA Kit, wu:fb68b07 ELISA Kit, zgc:109836 ELISA Kit, mannose binding lectin 2 ELISA Kit, Mannose-binding protein C ELISA Kit, mannose-binding lectin (protein C) 2, soluble ELISA Kit, mannose-binding lectin (protein C) 2 ELISA Kit, mannose-binding lectin family member 3, pseudogene ELISA Kit, MBL2 ELISA Kit, mbl2 ELISA Kit, Mbl2 ELISA Kit, MBL3P ELISA Kit
- Background
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Gene Name: Mannose Binding Lectin
Gene Aliases: MBL2, COLEC1, HSMBPC, MBP1, MBP, Collectin-1, Mannose-binding protein C, Mannan Binding Protein, Mannose-Binding Lectin(Protein C)2,Soluble(Opsonic Defect)
- Gene ID
- 4153
- UniProt
- P11226
- Pathways
- Complement System, Positive Regulation of Immune Effector Process
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