MMP7 CLIA Kit

Catalog No. ABIN5656780

Product Details MMP7 CLIA Kit

Target: MMP7 (Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7))

Reactivity: Rat

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 156.25 pg/mL - 10000 pg/mL

Minimum Detection Limit: 156.25 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 7 (MMP7). No significant cross-reactivity or interference between Matrix Metalloproteinase 7 (MMP7) and analogues was observed.

Sensitivity: 6.29 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 7 (MMP7). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 7 (MMP7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Matrix Metalloproteinase 7 (MMP7) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 7 (MMP7) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 7 (MMP7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: MMP7 (Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7))

Alternative Name: Matrix Metalloproteinase 7 (MMP7 Products)

Synonyms: MAT CLIA Kit, MPMM CLIA Kit, MMP-7 CLIA Kit, MPSL1 CLIA Kit, PUMP-1 CLIA Kit, matrilysin CLIA Kit, LOC727698 CLIA Kit, MMP7 CLIA Kit, Matrilysin CLIA Kit, matrix metallopeptidase 7 CLIA Kit, MMP7 CLIA Kit, Mmp7 CLIA Kit

Background:

Gene Name: Matrix Metalloproteinase 7

Gene Aliases: MPSL1, MAT, PUMP-1, Matrilysin Uterine, Matrilysin, Matrin, Pump-1 Protease, Uterine Metalloproteinase

Gene ID: 25335

UniProt: P50280

Pathways: Production of Molecular Mediator of Immune Response