Melanoma gp100 ELISA Kit

Catalog No. ABIN5656810

Product Details Melanoma gp100 ELISA Kit

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Melanoma Associated ME20 (ME20M). No significant cross-reactivity or interference between Melanoma Associated ME20 (ME20M) and analogues was observed.

Sensitivity: 0.053 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Melanoma Associated ME20 (ME20M). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Melanoma Associated ME20 (ME20M). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Melanoma Associated ME20 (ME20M), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Melanoma Associated ME20 (ME20M) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Melanoma Associated ME20 (ME20M) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Melanoma Associated ME20 (ME20M) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Melanoma gp100

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Alternative Name: Melanoma Associated ME20 (PMEL Products)

Synonyms: D12S53E ELISA Kit, ME20 ELISA Kit, ME20-M ELISA Kit, ME20M ELISA Kit, P1 ELISA Kit, P100 ELISA Kit, PMEL17 ELISA Kit, SI ELISA Kit, SIL ELISA Kit, SILV ELISA Kit, gp100 ELISA Kit, D10H12S53E ELISA Kit, D12S53Eh ELISA Kit, Pmel17 ELISA Kit, Si ELISA Kit, Silv ELISA Kit, gp87 ELISA Kit, RPE1 ELISA Kit, MMP115 ELISA Kit, silverb ELISA Kit, silver ELISA Kit, cb397 ELISA Kit, fdv ELISA Kit, pmel17 ELISA Kit, sb:cb397 ELISA Kit, silva ELISA Kit, wu:fc11g11 ELISA Kit, wu:fj24g11 ELISA Kit, zgc:136622 ELISA Kit, premelanosome protein ELISA Kit, premelanosome protein b ELISA Kit, premelanosome protein a ELISA Kit, PMEL ELISA Kit, Pmel ELISA Kit, pmelb ELISA Kit, pmela ELISA Kit

Background:

Gene Name: Melanoma Associated ME20

Gene Aliases: PMEL, GP100, SI, SIL, SILV, Pmel17, Silver Homolog, Premelanosome Protein, Silver Locus Protein Homolog, 95 kDa melanocyte-specific secreted glycoprotein

Gene ID: 6490

UniProt: P40967