MTTP ELISA Kit

Catalog No. ABIN5656885

Product Details MTTP ELISA Kit

Target: MTTP (Microsomal Triglyceride Transfer Protein (MTTP))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Microsomal Triglyceride Transfer Protein (MTTP). No significant cross-reactivity or interference between Microsomal Triglyceride Transfer Protein (MTTP) and analogues was observed.

Sensitivity: 0.056 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Microsomal Triglyceride Transfer Protein (MTTP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Microsomal Triglyceride Transfer Protein (MTTP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Microsomal Triglyceride Transfer Protein (MTTP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Microsomal Triglyceride Transfer Protein (MTTP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Microsomal Triglyceride Transfer Protein (MTTP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Microsomal Triglyceride Transfer Protein (MTTP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for MTTP

Target: MTTP (Microsomal Triglyceride Transfer Protein (MTTP))

Alternative Name: Microsomal Triglyceride Transfer Protein (MTTP Products)

Synonyms: ABL ELISA Kit, MTP ELISA Kit, 1810043K16Rik ELISA Kit, CG9342 ELISA Kit, CT3751 ELISA Kit, Dmel\\CG9342 ELISA Kit, dMTP ELISA Kit, wu:fd36b01 ELISA Kit, zgc:111876 ELISA Kit, microsomal triglyceride transfer protein ELISA Kit, Microsomal triacylglycerol transfer protein ELISA Kit, MTTP ELISA Kit, Mttp ELISA Kit, Mtp ELISA Kit, mttp ELISA Kit

Background:

Gene Name: Microsomal Triglyceride Transfer Protein

Gene Aliases: MTP, ABL, Microsomal triglyceride transfer protein large subunit

Gene ID: 17777

UniProt: O08601

Pathways: Transition Metal Ion Homeostasis