MAP1LC3A ELISA Kit

Catalog No. ABIN5656887

Product Details MAP1LC3A ELISA Kit

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a). No significant cross-reactivity or interference between Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a) and analogues was observed.

Sensitivity: 0.59 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for MAP1LC3A

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Alternative Name: Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3A Products)

Synonyms: ATG8E ELISA Kit, LC3 ELISA Kit, LC3A ELISA Kit, MAP1ALC3 ELISA Kit, MAP1BLC3 ELISA Kit, 1010001H21Rik ELISA Kit, 4922501H04Rik ELISA Kit, LC3a ELISA Kit, MGC69006 ELISA Kit, zgc:77094 ELISA Kit, MGC89867 ELISA Kit, MAP1LC3A ELISA Kit, map1lc3a ELISA Kit, microtubule associated protein 1 light chain 3 alpha ELISA Kit, microtubule-associated protein 1 light chain 3 alpha ELISA Kit, microtubule associated protein 1 light chain 3 alpha S homeolog ELISA Kit, MAP1LC3A ELISA Kit, Map1lc3a ELISA Kit, map1lc3a.S ELISA Kit, map1lc3a ELISA Kit

Background:

Gene Name: Microtubule Associated Protein 1 Light Chain 3 Alpha

Gene Aliases: MAP1ALC3, MAP1BLC3, LC3, LC3A, ATG8E, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1

Gene ID: 84557

UniProt: Q9H492

Pathways: Autophagy