Moesin ELISA Kit

Catalog No. ABIN5656931

Product Details Moesin ELISA Kit

Target: Moesin (MSN)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Moesin (MSN). No significant cross-reactivity or interference between Moesin (MSN) and analogues was observed.

Sensitivity: 0.055 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Moesin (MSN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Moesin (MSN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Moesin (MSN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Moesin (MSN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Moesin (MSN) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Moesin (MSN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Moesin

Target: Moesin (MSN)

Alternative Name: Moesin (MSN Products)

Synonyms: CG10701 ELISA Kit, D17 ELISA Kit, Dmel\\CG10701 ELISA Kit, Dmoe ELISA Kit, EMR1 ELISA Kit, ERM ELISA Kit, Emr1 ELISA Kit, anon-WO03040301.155 ELISA Kit, anon-WO03040301.157 ELISA Kit, anon-WO03040301.159 ELISA Kit, anon-WO03040301.161 ELISA Kit, dMoe ELISA Kit, gma ELISA Kit, l(1)G0067 ELISA Kit, l(1)G0323 ELISA Kit, l(1)G0404 ELISA Kit, l(1)G0415 ELISA Kit, moe ELISA Kit, moesin/ezrin/radixin homolog mRNA ELISA Kit, cb567 ELISA Kit, rdx ELISA Kit, wu:fb14b03 ELISA Kit, zgc:85640 ELISA Kit, MSN ELISA Kit, C78546 ELISA Kit, moesin ELISA Kit, Moesin ELISA Kit, moesin a ELISA Kit, moesin L homeolog ELISA Kit, MSN ELISA Kit, Moe ELISA Kit, msna ELISA Kit, msn.L ELISA Kit, moes ELISA Kit, Msn ELISA Kit

Background:

Gene Name: Moesin

Gene Aliases: Membrane-Organizing Extension Spike Protein

Gene ID: 4478

UniProt: P26038

Pathways: Asymmetric Protein Localization