MAX ELISA Kit

Catalog No. ABIN5657025

Product Details MAX ELISA Kit

Target: MAX (MYC Associated Factor X (MAX))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of MYC Associated Factor X (MAX). No significant cross-reactivity or interference between MYC Associated Factor X (MAX) and analogues was observed.

Sensitivity: 0.059 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to MYC Associated Factor X (MAX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to MYC Associated Factor X (MAX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MYC Associated Factor X (MAX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MYC Associated Factor X (MAX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MYC Associated Factor X (MAX) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MYC Associated Factor X (MAX) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for MAX

Target: MAX (MYC Associated Factor X (MAX))

Alternative Name: MYC Associated Factor X (MAX Products)

Synonyms: CG9648 ELISA Kit, Dmel\\CG9648 ELISA Kit, MAX ELISA Kit, bHLHd9 ELISA Kit, dMax ELISA Kit, dmax ELISA Kit, max ELISA Kit, bhlhd4 ELISA Kit, bhlhd5 ELISA Kit, bhlhd6 ELISA Kit, bhlhd7 ELISA Kit, bhlhd8 ELISA Kit, xmax2 ELISA Kit, xmax4 ELISA Kit, xmax ELISA Kit, AA960152 ELISA Kit, AI875693 ELISA Kit, bHLHd4 ELISA Kit, bHLHd5 ELISA Kit, bHLHd6 ELISA Kit, bHLHd7 ELISA Kit, bHLHd8 ELISA Kit, fa92b04 ELISA Kit, fb52a04 ELISA Kit, fb95h11 ELISA Kit, wu:fa92b04 ELISA Kit, wu:fb52a04 ELISA Kit, wu:fb95h11 ELISA Kit, zmax ELISA Kit, CG9648 gene product from transcript CG9648-RD ELISA Kit, MYC associated factor X L homeolog ELISA Kit, MYC associated factor X ELISA Kit, Max protein ELISA Kit, MYC associated factor X S homeolog ELISA Kit, myc associated factor X ELISA Kit, Max ELISA Kit, max.L ELISA Kit, MAX ELISA Kit, max ELISA Kit, max.S ELISA Kit

Background:

Gene Name: MYC Associated Factor X

Gene Aliases: BHLHD4, Class D basic helix-loop-helix protein 4

Gene ID: 4149

UniProt: P61244

Pathways: Mitotic G1-G1/S Phases