MOG ELISA Kit
Quick Overview for MOG ELISA Kit (ABIN5657038)
Target
See all MOG ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 3.12 ng/mL
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Purpose
- Human Myelin Oligodendrocyte Glycoprotein (MOG) Anti-antigen ELISA Kit for detecting MOG in serum, plasma
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Analytical Method
- Quantitative
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Specificity
- This assay has high sensitivity and excellent specificity for detection of Myelin Oligodendrocyte Glycoprotein (MOG). No significant cross-reactivity or interference between Myelin Oligodendrocyte Glycoprotein (MOG) and analogues was observed.
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Sensitivity
- 1.21 ng/mL
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Components
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Material not included
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Sample Volume
- 100 μL
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Assay Time
- 2.5 h
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Plate
- Pre-coated
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Assay Precision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myelin Oligodendrocyte Glycoprotein (MOG) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myelin Oligodendrocyte Glycoprotein (MOG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% -
Restrictions
- For Research Use only
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Handling Advice
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
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Storage
- 4 °C,-20 °C
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Storage Comment
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
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Expiry Date
- 12 months
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- MOG (Myelin Oligodendrocyte Glycoprotein (MOG))
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Alternative Name
- Myelin Oligodendrocyte Glycoprotein
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Background
- Gene Aliases:
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Gene ID
- 4340
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UniProt
- Q16653
Target See all MOG ELISA Kits
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