MYH10 ELISA Kit

Catalog No. ABIN5657087

Product Details MYH10 ELISA Kit

Target: MYH10 (Myosin, Heavy Polypeptide 10, Non-Muscle (MYH10))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Myosin Heavy Chain 10, Non Muscle (MYH10). No significant cross-reactivity or interference between Myosin Heavy Chain 10, Non Muscle (MYH10) and analogues was observed.

Sensitivity: 0.055 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myosin Heavy Chain 10, Non Muscle (MYH10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myosin Heavy Chain 10, Non Muscle (MYH10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myosin Heavy Chain 10, Non Muscle (MYH10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myosin Heavy Chain 10, Non Muscle (MYH10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myosin Heavy Chain 10, Non Muscle (MYH10) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myosin Heavy Chain 10, Non Muscle (MYH10) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for MYH10

Target: MYH10 (Myosin, Heavy Polypeptide 10, Non-Muscle (MYH10))

Alternative Name: Myosin Heavy Chain 10, Non Muscle (MYH10 Products)

Synonyms: 5730504C04Rik ELISA Kit, 9330167F11Rik ELISA Kit, Fltn ELISA Kit, Myhn-2 ELISA Kit, Myhn2 ELISA Kit, NMHC II-B ELISA Kit, NMHC-B ELISA Kit, NMHCII-B ELISA Kit, NMMHC II-b ELISA Kit, NMMHC-B ELISA Kit, NMMHC-IIB ELISA Kit, SMemb ELISA Kit, mKIAA3005 ELISA Kit, MCH-B ELISA Kit, nmmhcb ELISA Kit, myosin ELISA Kit, myosin-10 ELISA Kit, non-muscle ELISA Kit, NMMHCB ELISA Kit, dZ204D19.2 ELISA Kit, si:dz150i12.3 ELISA Kit, wu:fc46h07 ELISA Kit, wu:fy19d11 ELISA Kit, myosin, heavy polypeptide 10, non-muscle ELISA Kit, myosin heavy chain 10 ELISA Kit, myosin, heavy chain 10, non-muscle S homeolog ELISA Kit, myosin, heavy chain 10, non-muscle ELISA Kit, Myh10 ELISA Kit, myh10.S ELISA Kit, MYH10 ELISA Kit, myh10 ELISA Kit

Background:

Gene Name: Myosin Heavy Chain 10, Non Muscle

Gene Aliases: NMMHCB, Cellular myosin heavy chain, type B, Myosin heavy chain, non-muscle IIb

Gene ID: 4628

UniProt: P35580