MYL3/CMLC1 ELISA Kit

Catalog No. ABIN5657105

Product Details MYL3/CMLC1 ELISA Kit

Target: MYL3/CMLC1 (MYL3) (Myosin, Light Chain 3 (MYL3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78.12 pg/mL - 5000 pg/mL

Minimum Detection Limit: 78.12 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Myosin Light Chain 3, Alkali, Ventricular, Slow Skeletal (MYL3). No significant cross-reactivity or interference between Myosin Light Chain 3, Alkali, Ventricular, Slow Skeletal (MYL3) and analogues was observed.

Sensitivity: 30 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myosin Light Chain 3, Alkali, Ventricular, Slow Skeletal (MYL3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myosin Light Chain 3, Alkali, Ventricular, Slow Skeletal (MYL3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myosin Light Chain 3, Alkali, Ventricular, Slow Skeletal (MYL3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myosin Light Chain 3, Alkali, Ventricular, Slow Skeletal (MYL3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myosin Light Chain 3, Alkali, Ventricular, Slow Skeletal (MYL3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myosin Light Chain 3, Alkali, Ventricular, Slow Skeletal (MYL3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for MYL3/CMLC1

Target: MYL3/CMLC1 (MYL3) (Myosin, Light Chain 3 (MYL3))

Alternative Name: Myosin Light Chain 3, Alkali, Ventricular, Slow Skeletal (MYL3 Products)

Synonyms: CMD1S ELISA Kit, CMH1 ELISA Kit, MPD1 ELISA Kit, MYHCB ELISA Kit, SPMD ELISA Kit, SPMM ELISA Kit, B-MHC ELISA Kit, MyHC-I ELISA Kit, Myhc-b ELISA Kit, Myhcb ELISA Kit, beta-MHC ELISA Kit, MLC1s ELISA Kit, MLC1v ELISA Kit, Mylc ELISA Kit, VLC1 ELISA Kit, Mylc1v ELISA Kit, CMH8 ELISA Kit, MLC1SB ELISA Kit, MLC1V ELISA Kit, mlc1v ELISA Kit, myl3-a ELISA Kit, myl3-b ELISA Kit, myl3.L ELISA Kit, zgc:103441 ELISA Kit, myosin heavy chain 7 ELISA Kit, myosin, heavy polypeptide 7, cardiac muscle, beta ELISA Kit, myosin, light polypeptide 3 ELISA Kit, myosin light chain 3 ELISA Kit, myosin light chain 3 S homeolog ELISA Kit, cardiac myosin light chain-1 ELISA Kit, MYH7 ELISA Kit, Myh7 ELISA Kit, Myl3 ELISA Kit, MYL3 ELISA Kit, myl3.S ELISA Kit, cmlc1 ELISA Kit

Background:

Gene Name: Myosin Light Chain 3, Alkali, Ventricular, Slow Skeletal

Gene Aliases: CMH8, MLC1V, VLC1, MLC1SB, Cardiac myosin light chain 1, Ventricular/slow twitch myosin alkali light chain

Gene ID: 4634

UniProt: P08590