Nerve Growth Factor CLIA Kit

Catalog No. ABIN5657224

The Rat Nerve Growth Factor ELISA Kit (ABIN5657224) is a Chemiluminescent ELISA Kit designed to quantify Rat Nerve Growth Factor.

Quick Overview for Nerve Growth Factor CLIA Kit (ABIN5657224)

Target: Nerve Growth Factor (NGF)

Reactivity: Rat

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 8.23 pg/mL - 6000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Product Details Nerve Growth Factor CLIA Kit

Minimum Detection Limit: 8.23 pg/mL

Purpose: Rat Nerve Growth Factor (NGF) CLIA Kit for detecting NGF in serum, plasma, tissue homogenates, cell lysates, cell culture supernates

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Nerve Growth Factor (NGF). No significant cross-reactivity or interference between Nerve Growth Factor (NGF) and analogues was observed.

Sensitivity: 3.13 pg/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• Substrate A
• Substrate B
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Nerve Growth Factor (NGF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Nerve Growth Factor (NGF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Nerve Growth Factor (NGF) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nerve Growth Factor (NGF) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nerve Growth Factor (NGF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: Nerve Growth Factor (NGF)

Alternative Name: Nerve Growth Factor

Background: Gene Aliases: NGFB, Beta-NGF, HSAN5, NGF-B, Beta-Nerve Growth Factor

Gene ID: 310738

UniProt: P25427

Pathways: Regulation of Cell Size