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Neuropilin 1 CLIA Kit

NRP1 Reactivity: Human Chemiluminescent Sandwich ELISA 312.5 pg/mL - 20000 pg/mL Plasma, Serum, Tissue Homogenate
Catalog No. ABIN5657326
  • Target See all Neuropilin 1 (NRP1) CLIA Kits
    Neuropilin 1 (NRP1)
    Reactivity
    • 6
    • 5
    • 3
    • 1
    Human
    Detection Method
    Chemiluminescent
    Method Type
    Sandwich ELISA
    Detection Range
    312.5 pg/mL - 20000 pg/mL
    Minimum Detection Limit
    312.5 pg/mL
    Application
    ELISA
    Sample Type
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Neuropilin 1 (NRP1). No significant cross-reactivity or interference between Neuropilin 1 (NRP1) and analogues was observed.
    Sensitivity
    11.45 pg/mL
    Top Product
    Discover our top product NRP1 ELISA Kit
  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    2 - 3 h
    Plate
    Pre-coated
    Protocol
    The microplate provided in this kit has been pre-coated with an antibody specific to Neuropilin 1 (NRP1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Neuropilin 1 (NRP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Neuropilin 1 (NRP1) level in the sample or standard.,
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuropilin 1 (NRP1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuropilin 1 (NRP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all Neuropilin 1 (NRP1) CLIA Kits
    Neuropilin 1 (NRP1)
    Alternative Name
    Neuropilin 1 (NRP1 Products)
    Synonyms
    NRP1 CLIA Kit, cd304 CLIA Kit, neuropilin CLIA Kit, np-1 CLIA Kit, npn-1 CLIA Kit, nrp CLIA Kit, nrp-1 CLIA Kit, vegf165r CLIA Kit, BDCA4 CLIA Kit, CD304 CLIA Kit, NP1 CLIA Kit, NRP CLIA Kit, VEGF165R CLIA Kit, C530029I03 CLIA Kit, NP-1 CLIA Kit, NPN-1 CLIA Kit, Npn1 CLIA Kit, Nrp CLIA Kit, neuropilin 1 CLIA Kit, neuropilin 1 L homeolog CLIA Kit, NRP1 CLIA Kit, nrp1 CLIA Kit, Nrp1 CLIA Kit, nrp1.L CLIA Kit
    Background

    Gene Name: Neuropilin 1

    Gene Aliases: CD304, BDCA4, VEGF165R, Vascular endothelial cell growth factor 165 receptor

    Gene ID
    8829
    UniProt
    O14786
    Pathways
    Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals
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