NOS2 ELISA Kit

Catalog No. ABIN5657405

Product Details NOS2 ELISA Kit

Target: NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Nitric Oxide Synthase 2, Inducible (NOS2). No significant cross-reactivity or interference between Nitric Oxide Synthase 2, Inducible (NOS2) and analogues was observed.

Sensitivity: 0.62 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nitric Oxide Synthase 2, Inducible (NOS2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nitric Oxide Synthase 2, Inducible (NOS2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nitric Oxide Synthase 2, Inducible (NOS2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nitric Oxide Synthase 2, Inducible (NOS2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nitric Oxide Synthase 2, Inducible (NOS2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nitric Oxide Synthase 2, Inducible (NOS2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for NOS2

Target: NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))

Alternative Name: Nitric Oxide Synthase 2, Inducible (NOS2 Products)

Synonyms: HEP-NOS ELISA Kit, INOS ELISA Kit, NOS ELISA Kit, NOS2A ELISA Kit, NOS-II ELISA Kit, Nos-2 ELISA Kit, Nos2a ELISA Kit, i-NOS ELISA Kit, iNOS ELISA Kit, iNos ELISA Kit, NOS2a ELISA Kit, NOS2 ELISA Kit, BmNOS2 ELISA Kit, Nsl ELISA Kit, nos2 ELISA Kit, nitric oxide synthase 2 ELISA Kit, nitric oxide synthase 2, inducible ELISA Kit, inducible nitric oxide synthase ELISA Kit, inducible nitric-oxide synthase ELISA Kit, nanos C2HC-type zinc finger 2 ELISA Kit, NOS2 ELISA Kit, Nos2 ELISA Kit, nos2 ELISA Kit, LOC396821 ELISA Kit, NANOS2 ELISA Kit

Background:

Gene Name: Nitric Oxide Synthase 2, Inducible

Gene Aliases: NOS2A, INOS, HEP-NOS, I-NOS, Hepatocytes Oxide Synthase, Peptidyl-cysteine S-nitrosylase NOS2

Gene ID: 24599

UniProt: Q06518

Pathways: Retinoic Acid Receptor Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Inositol Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process