ODC1 ELISA Kit

Catalog No. ABIN5657525

Product Details ODC1 ELISA Kit

Target: ODC1 (Ornithine Decarboxylase 1 (ODC1))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Ornithine Decarboxylase (ODC). No significant cross-reactivity or interference between Ornithine Decarboxylase (ODC) and analogues was observed.

Sensitivity: 0.059 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ornithine Decarboxylase (ODC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ornithine Decarboxylase (ODC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ornithine Decarboxylase (ODC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ornithine Decarboxylase (ODC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ornithine Decarboxylase (ODC) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ornithine Decarboxylase (ODC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for ODC1

Target: ODC1 (Ornithine Decarboxylase 1 (ODC1))

Alternative Name: Ornithine Decarboxylase (ODC1 Products)

Synonyms: CG8721 ELISA Kit, Dmel\\CG8721 ELISA Kit, dodc1 ELISA Kit, odc ELISA Kit, fc54f04 ELISA Kit, fi06d08 ELISA Kit, CHUNP6922 ELISA Kit, wu:fc54f04 ELISA Kit, wu:fi06d08 ELISA Kit, odc2 ELISA Kit, xODC1 ELISA Kit, xodc2 ELISA Kit, DDBDRAFT_0204020 ELISA Kit, DDBDRAFT_0237755 ELISA Kit, DDB_0204020 ELISA Kit, DDB_0237755 ELISA Kit, ODC ELISA Kit, odc1 ELISA Kit, ODC1 ELISA Kit, Odc ELISA Kit, odc1-a ELISA Kit, Ornithine decarboxylase 1 ELISA Kit, ornithine decarboxylase 1 ELISA Kit, ornithine decarboxylase ELISA Kit, diaminopimelate decarboxylase ELISA Kit, Orn/DAP/Arg decarboxylase 2 domain-containing protein ELISA Kit, Diaminopimelate decarboxylase ELISA Kit, Ornithine decarboxylase ELISA Kit, ornithine decarboxylase 1 L homeolog ELISA Kit, ornithine decarboxylase, structural 1 ELISA Kit, Odc1 ELISA Kit, odc1 ELISA Kit, ODC1 ELISA Kit, odc ELISA Kit, RR_RS04895 ELISA Kit, ASA_RS06380 ELISA Kit, LOC100232922 ELISA Kit, odc1.L ELISA Kit, odc-1 ELISA Kit

Background:

Gene Name: Ornithine Decarboxylase

Gene Aliases: ODC1

Gene ID: 24609

UniProt: P09057