PEX2 ELISA Kit
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- Target See all PEX2 ELISA Kits
- PEX2 (Peroxisomal Biogenesis Factor 2 (PEX2))
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.156 ng/mL - 10 ng/mL
- Minimum Detection Limit
- 0.156 ng/mL
- Application
- ELISA
- Sample Type
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificity
- This assay has high sensitivity and excellent specificity for detection of Peroxisomal Biogenesis Factor 2 (PEX2). No significant cross-reactivity or interference between Peroxisomal Biogenesis Factor 2 (PEX2) and analogues was observed.
- Sensitivity
- 0.056 ng/mL
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- Comment
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Assay Time
- 3 h
- Plate
- Pre-coated
- Protocol
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxisomal Biogenesis Factor 2 (PEX2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peroxisomal Biogenesis Factor 2 (PEX2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peroxisomal Biogenesis Factor 2 (PEX2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Peroxisomal Biogenesis Factor 2 (PEX2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Assay Precision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Peroxisomal Biogenesis Factor 2 (PEX2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Peroxisomal Biogenesis Factor 2 (PEX2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Handling Advice
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Storage
- 4 °C,-20 °C
- Storage Comment
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Expiry Date
- 4-8 months
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- Target See all PEX2 ELISA Kits
- PEX2 (Peroxisomal Biogenesis Factor 2 (PEX2))
- Alternative Name
- Peroxisomal Biogenesis Factor 2 (PEX2 Products)
- Synonyms
- PAF1 ELISA Kit, PBD5A ELISA Kit, PBD5B ELISA Kit, PMP3 ELISA Kit, PMP35 ELISA Kit, PXMP3 ELISA Kit, RNF72 ELISA Kit, ZWS3 ELISA Kit, D3Ertd138e ELISA Kit, PAF-1 ELISA Kit, Pxmp3 ELISA Kit, pxmp3 ELISA Kit, An18g04150 ELISA Kit, DDBDRAFT_0168812 ELISA Kit, DDBDRAFT_0238045 ELISA Kit, DDB_0168812 ELISA Kit, DDB_0238045 ELISA Kit, peroxisomal biogenesis factor 2 ELISA Kit, RING zinc finger-containing protein ELISA Kit, PEX2 ELISA Kit, Pex2 ELISA Kit, pex2 ELISA Kit, ANI_1_1336164 ELISA Kit, HCAG_04580 ELISA Kit, CC1G_00418 ELISA Kit, PTRG_10616 ELISA Kit, PAAG_05772 ELISA Kit, MGYG_06097 ELISA Kit
- Background
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Gene Name: Peroxisomal Biogenesis Factor 2
Gene Aliases: PXMP3, PAF1, PMP35, PMP3, RNF72, Peroxin 2, Peroxisome Assembly Factor 1, Peroxisomal Membrane Protein 35 kDa, Peroxisomal Membrane Protein 3
- Gene ID
- 5828
- UniProt
- P28328
- Pathways
- Monocarboxylic Acid Catabolic Process
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