PI4KA ELISA Kit

Catalog No. ABIN5657761

Product Details PI4KA ELISA Kit

Target: PI4KA (Phosphatidylinositol 4-Kinase, Catalytic, alpha (PI4KA))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78 ng/mL - 50 ng/mL

Minimum Detection Limit: 0.78 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Phosphatidylinositol-4-Kinase Catalytic Alpha (PI4Ka). No significant cross-reactivity or interference between Phosphatidylinositol-4-Kinase Catalytic Alpha (PI4Ka) and analogues was observed.

Sensitivity: 0.27 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphatidylinositol-4-Kinase Catalytic Alpha (PI4Ka). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphatidylinositol-4-Kinase Catalytic Alpha (PI4Ka). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphatidylinositol-4-Kinase Catalytic Alpha (PI4Ka), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphatidylinositol-4-Kinase Catalytic Alpha (PI4Ka) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phosphatidylinositol-4-Kinase Catalytic Alpha (PI4Ka) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phosphatidylinositol-4-Kinase Catalytic Alpha (PI4Ka) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for PI4KA

Target: PI4KA (Phosphatidylinositol 4-Kinase, Catalytic, alpha (PI4KA))

Alternative Name: Phosphatidylinositol-4-Kinase Catalytic Alpha (PI4KA Products)

Synonyms: PI4K-ALPHA ELISA Kit, PIK4CA ELISA Kit, pi4K230 ELISA Kit, KAL ELISA Kit, KLST ELISA Kit, KST ELISA Kit, PI-4 ELISA Kit, PI4 ELISA Kit, kallistatin ELISA Kit, Pik4ca ELISA Kit, PI4-kinase alpha ELISA Kit, PI4K-alpha ELISA Kit, PtdIns-4-kinase alpha ELISA Kit, zgc:172062 ELISA Kit, pi4ka ELISA Kit, pik4ca ELISA Kit, phosphatidylinositol 4-kinase alpha ELISA Kit, serpin family A member 4 ELISA Kit, phosphatidylinositol 4-kinase, catalytic, alpha b ELISA Kit, phosphatidylinositol 4-kinase, catalytic, alpha S homeolog ELISA Kit, PI4KA ELISA Kit, SERPINA4 ELISA Kit, Pi4ka ELISA Kit, pi4kab ELISA Kit, pi4ka.S ELISA Kit

Background:

Gene Name: Phosphatidylinositol-4-Kinase Catalytic Alpha

Gene Aliases: PIK4CA, PI4K-ALPHA, pi4K230

Gene ID: 5297

UniProt: P42356

Pathways: Inositol Metabolic Process