PGK1 ELISA Kit

Catalog No. ABIN5657778

Product Details PGK1 ELISA Kit

Target: PGK1 (Phosphoglycerate Kinase 1 (PGK1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.25 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.25 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Phosphoglycerate Kinase 1 (PGK1). No significant cross-reactivity or interference between Phosphoglycerate Kinase 1 (PGK1) and analogues was observed.

Sensitivity: 12.9 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphoglycerate Kinase 1 (PGK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphoglycerate Kinase 1 (PGK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphoglycerate Kinase 1 (PGK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphoglycerate Kinase 1 (PGK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phosphoglycerate Kinase 1 (PGK1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phosphoglycerate Kinase 1 (PGK1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for PGK1

Target: PGK1 (Phosphoglycerate Kinase 1 (PGK1))

Alternative Name: Phosphoglycerate Kinase 1 (PGK1 Products)

Synonyms: MIG10 ELISA Kit, PGKA ELISA Kit, Pgk-1 ELISA Kit, Pgk ELISA Kit, PGK ELISA Kit, wu:fd59b07 ELISA Kit, wu:fj36g06 ELISA Kit, zgc:56252 ELISA Kit, zgc:77899 ELISA Kit, PGK1 ELISA Kit, phosphoglycerate kinase 1 ELISA Kit, pgk2 ELISA Kit, pgka ELISA Kit, phosphoglycerate kinase 1 ELISA Kit, phosphoglycerate kinase 1 L homeolog ELISA Kit, PGK1 ELISA Kit, Pgk1 ELISA Kit, pgk1 ELISA Kit, LOC100348124 ELISA Kit, pgk1.L ELISA Kit

Background:

Gene Name: Phosphoglycerate Kinase 1

Gene Aliases: MIG10, PGKA, PRP 2, Cell migration-inducing gene 10 protein, Primer recognition protein 2

Gene ID: 5230

UniProt: P00558

Pathways: Cellular Glucan Metabolic Process