PGAM1 ELISA Kit

Catalog No. ABIN5657779

Product Details PGAM1 ELISA Kit

Target: PGAM1 (phosphoglycerate Mutase 1 (Brain) (PGAM1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Phosphoglycerate Mutase 1, Brain (PGAM1). No significant cross-reactivity or interference between Phosphoglycerate Mutase 1, Brain (PGAM1) and analogues was observed.

Sensitivity: 0.58 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphoglycerate Mutase 1, Brain (PGAM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphoglycerate Mutase 1, Brain (PGAM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphoglycerate Mutase 1, Brain (PGAM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphoglycerate Mutase 1, Brain (PGAM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phosphoglycerate Mutase 1, Brain (PGAM1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phosphoglycerate Mutase 1, Brain (PGAM1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for PGAM1

Target: PGAM1 (phosphoglycerate Mutase 1 (Brain) (PGAM1))

Alternative Name: Phosphoglycerate Mutase 1, Brain (PGAM1 Products)

Synonyms: PGAM-B ELISA Kit, PGAMA ELISA Kit, Pgmut ELISA Kit, MGC81450 ELISA Kit, 2310050F24Rik ELISA Kit, Pgam-1 ELISA Kit, pgam1 ELISA Kit, wu:fb81h05 ELISA Kit, fd15c02 ELISA Kit, pgam1lb ELISA Kit, wu:fd15c02 ELISA Kit, zgc:63722 ELISA Kit, phosphoglycerate mutase 1 ELISA Kit, phosphoglycerate mutase 1 L homeolog ELISA Kit, phosphoglycerate mutase 1a ELISA Kit, phosphoglycerate mutase 1b ELISA Kit, PGAM1 ELISA Kit, Pgam1 ELISA Kit, pgam1.L ELISA Kit, pgam1 ELISA Kit, pgam1a ELISA Kit, pgam1b ELISA Kit

Background:

Gene Name: Phosphoglycerate Mutase 1, Brain

Gene Aliases: PGAMA, PGAM-B, BPG-dependent PGAM 1, Phosphoglycerate mutase isozyme B

Gene ID: 5223

UniProt: P18669

Pathways: Regulation of Carbohydrate Metabolic Process