PIK3C2A ELISA Kit

Catalog No. ABIN5657785

Product Details PIK3C2A ELISA Kit

Target: PIK3C2A (Phosphoinositide-3-Kinase, Class 2, alpha Polypeptide (PIK3C2A))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide (PIK3C2a). No significant cross-reactivity or interference between Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide (PIK3C2a) and analogues was observed.

Sensitivity: 0.057 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide (PIK3C2a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide (PIK3C2a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide (PIK3C2a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide (PIK3C2a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide (PIK3C2a) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide (PIK3C2a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for PIK3C2A

Target: PIK3C2A (Phosphoinositide-3-Kinase, Class 2, alpha Polypeptide (PIK3C2A))

Alternative Name: Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide (PIK3C2A Products)

Synonyms: CPK ELISA Kit, PI3-K-C2(ALPHA) ELISA Kit, PI3-K-C2A ELISA Kit, cpk ELISA Kit, pi3-k-c2a ELISA Kit, Cpk-m ELISA Kit, PI3KC2 ELISA Kit, sb:cb156 ELISA Kit, si:dkey-24l11.7 ELISA Kit, wu:fc02a04 ELISA Kit, phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha ELISA Kit, phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 alpha ELISA Kit, phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha S homeolog ELISA Kit, phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha ELISA Kit, PIK3C2A ELISA Kit, Pik3c2a ELISA Kit, pik3c2a ELISA Kit, pik3c2a.S ELISA Kit, LOC100548137 ELISA Kit

Background:

Gene Name: Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide

Gene Aliases: PI3K2a, CPK, PI3-K-C2(Alpha), PI3-K-C2A, PI3K2-a, Phosphoinositide 3-Kinase 2a, Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha

Gene ID: 18704

UniProt: Q61194

Pathways: EGFR Signaling Pathway, Inositol Metabolic Process, Platelet-derived growth Factor Receptor Signaling