PTTG1IP ELISA Kit

Catalog No. ABIN5657835

Product Details PTTG1IP ELISA Kit

Target: PTTG1IP (Pituitary Tumor-Transforming 1 Interacting Protein (PTTG1IP))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Pituitary Tumor Transforming 1 Interacting Protein (PTTG1IP). No significant cross-reactivity or interference between Pituitary Tumor Transforming 1 Interacting Protein (PTTG1IP) and analogues was observed.

Sensitivity: 0.071 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pituitary Tumor Transforming 1 Interacting Protein (PTTG1IP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pituitary Tumor Transforming 1 Interacting Protein (PTTG1IP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pituitary Tumor Transforming 1 Interacting Protein (PTTG1IP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pituitary Tumor Transforming 1 Interacting Protein (PTTG1IP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pituitary Tumor Transforming 1 Interacting Protein (PTTG1IP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pituitary Tumor Transforming 1 Interacting Protein (PTTG1IP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for PTTG1IP

Target: PTTG1IP (Pituitary Tumor-Transforming 1 Interacting Protein (PTTG1IP))

Alternative Name: Pituitary Tumor Transforming 1 Interacting Protein (PTTG1IP Products)

Synonyms: 1810010L20Rik ELISA Kit, AI314311 ELISA Kit, AU018448 ELISA Kit, C79540 ELISA Kit, C21orf1 ELISA Kit, C21orf3 ELISA Kit, PBF ELISA Kit, pttg1ip ELISA Kit, zgc:103708 ELISA Kit, pituitary tumor-transforming 1 interacting protein ELISA Kit, PTTG1 interacting protein ELISA Kit, PTTG1 interacting protein b ELISA Kit, Pttg1ip ELISA Kit, PTTG1IP ELISA Kit, pttg1ipb ELISA Kit

Background:

Gene Name: Pituitary Tumor Transforming 1 Interacting Protein

Gene Aliases: PBF, C21orf1, C21orf3, Pituitary tumor-transforming gene protein-binding factor

Gene ID: 754

UniProt: P53801

Pathways: Protein targeting to Nucleus