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PLEC ELISA Kit

PLEC Reactivity: Human Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Tissue Homogenate
Catalog No. ABIN5657944
  • Target See all PLEC ELISA Kits
    PLEC (Plectin (PLEC))
    Reactivity
    • 2
    • 2
    • 2
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    1.56 ng/mL - 100 ng/mL
    Minimum Detection Limit
    1.56 ng/mL
    Application
    ELISA
    Sample Type
    Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Plectin (PLEC). No significant cross-reactivity or interference between Plectin (PLEC) and analogues was observed.
    Sensitivity
    0.58 ng/mL
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  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Plectin (PLEC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Plectin (PLEC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Plectin (PLEC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Plectin (PLEC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Plectin (PLEC) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Plectin (PLEC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all PLEC ELISA Kits
    PLEC (Plectin (PLEC))
    Alternative Name
    Plectin (PLEC Products)
    Synonyms
    EBS1 ELISA Kit, EBSO ELISA Kit, HD1 ELISA Kit, LGMD2Q ELISA Kit, PCN ELISA Kit, PLEC1 ELISA Kit, PLEC1b ELISA Kit, PLTN ELISA Kit, AA591047 ELISA Kit, AU042537 ELISA Kit, Plec1 ELISA Kit, Pcn ELISA Kit, Pltn ELISA Kit, IFAP300 ELISA Kit, Plectin-1 ELISA Kit, plectin ELISA Kit, plectin L homeolog ELISA Kit, LOC100481602 ELISA Kit, plec.L ELISA Kit, PLEC ELISA Kit, Plec ELISA Kit, LOC786966 ELISA Kit
    Background

    Gene Name: Plectin

    Gene Aliases: HD1, PCN, EBS1, EBSO, PLEC1b, PLTN, Hemidesmosomal protein 1, Intermediate Filament Binding Protein 500 kDa, Epidermolysis Bullosa Simplex 1(Ogna)

    Gene ID
    5339
    UniProt
    Q15149
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