Progesterone CLIA Kit

Catalog No. ABIN5658065

Various Species Progesterone ELISA Kit, Chemiluminescent assay for quantification of Various Species Progesterone.

Quick Overview for Progesterone CLIA Kit (ABIN5658065)

Target: Progesterone

Reactivity: Various Species

Detection Method: Chemiluminescent

Method Type: Competition ELISA

Detection Range: 1.2 ng/mL - 300 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details Progesterone CLIA Kit

Minimum Detection Limit: 1.2 ng/mL

Purpose: Progesterone (PG) CLIA Kit for detecting PG in serum, plasma

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Progesterone (PG). No significant cross-reactivity or interference between Progesterone (PG) and analogues was observed.

Sensitivity: 0.35 ng/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• Substrate A
• Substrate B
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Sample Volume: 50 μL

Assay Time: 2 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Progesterone (PG). A competitive inhibition reaction is launched between biotin labeled Progesterone (PG) and unlabeled Progesterone (PG) (Standards or samples) with the pre-coated antibody specific to Progesterone (PG). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Progesterone (PG) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Progesterone (PG) level in the sample or standard.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Progesterone (PG) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Progesterone (PG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: Progesterone

Target Type: Hormone

Background: Gene Aliases: P4, Pregn-4-Ene-3,20-Dione