PROC ELISA Kit

Catalog No. ABIN5658189

Product Details PROC ELISA Kit

Target: PROC (Vitamin K-dependent protein C (PROC))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Minimum Detection Limit: 62.5 pg/mL

Application: ELISA

Sample Type: Plasma

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Protein C (PROC). No significant cross-reactivity or interference between Protein C (PROC) and analogues was observed.

Sensitivity: 24.8 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein C (PROC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein C (PROC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein C (PROC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein C (PROC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein C (PROC) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein C (PROC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for PROC

Target: PROC (Vitamin K-dependent protein C (PROC))

Alternative Name: Protein C (PROC Products)

Synonyms: proc ELISA Kit, si:ch1073-188c16.3 ELISA Kit, zgc:63987 ELISA Kit, PROC ELISA Kit, APC ELISA Kit, PC ELISA Kit, PROC1 ELISA Kit, THPH3 ELISA Kit, THPH4 ELISA Kit, proc1 ELISA Kit, MGC64425 ELISA Kit, PA ELISA Kit, protein C, inactivator of coagulation factors Va and VIIIa ELISA Kit, protein C (inactivator of coagulation factors Va and VIIIa), a ELISA Kit, protein C (inactivator of coagulation factors Va and VIIIa) ELISA Kit, protein C, inactivator of coagulation factors Va and VIIIa S homeolog ELISA Kit, vitamin K-dependent protein C ELISA Kit, prosaposin ELISA Kit, protein C ELISA Kit, proline rich protein HaeIII subfamily 1 ELISA Kit, PROC ELISA Kit, proca ELISA Kit, proc.S ELISA Kit, proc ELISA Kit, CpipJ_CPIJ000393 ELISA Kit, CpipJ_CPIJ002754 ELISA Kit, CpipJ_CPIJ003717 ELISA Kit, CpipJ_CPIJ003718 ELISA Kit, CpipJ_CPIJ014440 ELISA Kit, CpipJ_CPIJ018032 ELISA Kit, CpipJ_CPIJ018737 ELISA Kit, PSAP ELISA Kit, Proc ELISA Kit, PRH1 ELISA Kit

Target Type: Viral Protein

Background:

Gene Name: Protein C

Gene Aliases: PRO-C, VKDPC, PC, Vitamin K Dependent Protein C, Inactivator Of Coagulation Factors Va And VIIIa, Autoprothrombin IIA, Blood coagulation factor XIV, Activation peptide

Gene ID: 5624

UniProt: P04070