Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

PDIA6 ELISA Kit

PDIA6 Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Tissue Homogenate
Catalog No. ABIN5658205
  • Target See all PDIA6 ELISA Kits
    PDIA6 (Protein Disulfide Isomerase Family A, Member 6 (PDIA6))
    Reactivity
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.156 ng/mL - 10 ng/mL
    Minimum Detection Limit
    0.156 ng/mL
    Application
    ELISA
    Sample Type
    Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Protein Disulfide Isomerase A6 (PDIA6). No significant cross-reactivity or interference between Protein Disulfide Isomerase A6 (PDIA6) and analogues was observed.
    Sensitivity
    0.049 ng/mL
    Featured
    Discover our best selling PDIA6 ELISA Kit
    Top Product
    Discover our top product PDIA6 ELISA Kit
  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Disulfide Isomerase A6 (PDIA6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Disulfide Isomerase A6 (PDIA6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Disulfide Isomerase A6 (PDIA6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Disulfide Isomerase A6 (PDIA6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Disulfide Isomerase A6 (PDIA6) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Disulfide Isomerase A6 (PDIA6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all PDIA6 ELISA Kits
    PDIA6 (Protein Disulfide Isomerase Family A, Member 6 (PDIA6))
    Alternative Name
    Protein Disulfide Isomerase A6 (PDIA6 Products)
    Synonyms
    1700015E05Rik ELISA Kit, AL023058 ELISA Kit, C77895 ELISA Kit, CaBP5 ELISA Kit, P5 ELISA Kit, Txndc7 ELISA Kit, ERP5 ELISA Kit, TXNDC7 ELISA Kit, CaBP1 ELISA Kit, pdi-p5 ELISA Kit, erp5 ELISA Kit, pdia6 ELISA Kit, pdip5 ELISA Kit, txndc7 ELISA Kit, protein disulfide isomerase family A member 6 ELISA Kit, protein disulfide isomerase associated 6 ELISA Kit, protein disulfide isomerase family A, member 6 ELISA Kit, protein disulfide isomerase family A member 6 L homeolog ELISA Kit, PDIA6 ELISA Kit, Pdia6 ELISA Kit, pdia6.L ELISA Kit
    Background

    Gene Name: Protein Disulfide Isomerase A6

    Gene Aliases: PDI-A6, TXNDC7, P5, ERp5, Thioredoxin Domain Containing 7(Protein Disulfide Isomerase), Protein Disulfide Isomerase-Associated 6, Endoplasmic reticulum protein 5

    Gene ID
    10130
    UniProt
    Q15084
    Pathways
    ER-Nucleus Signaling, Cell RedoxHomeostasis
You are here:
Support