PKN1 ELISA Kit

Catalog No. ABIN5658228

Product Details PKN1 ELISA Kit

Target: PKN1 (Protein Kinase N1 (PKN1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Protein Kinase N1 (PKN1). No significant cross-reactivity or interference between Protein Kinase N1 (PKN1) and analogues was observed.

Sensitivity: 0.117 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase N1 (PKN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase N1 (PKN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase N1 (PKN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Kinase N1 (PKN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Kinase N1 (PKN1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Kinase N1 (PKN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for PKN1

Target: PKN1 (Protein Kinase N1 (PKN1))

Alternative Name: Protein Kinase N1 (PKN1 Products)

Synonyms: DBK ELISA Kit, PAK-1 ELISA Kit, PAK1 ELISA Kit, PKN ELISA Kit, PKN-ALPHA ELISA Kit, PRK1 ELISA Kit, PRKCL1 ELISA Kit, F730027O18Rik ELISA Kit, Pkn ELISA Kit, Prkcl1 ELISA Kit, Stk3 ELISA Kit, PKN1 ELISA Kit, 3-2 ELISA Kit, CG2049 ELISA Kit, CG2055 ELISA Kit, CT6637 ELISA Kit, CT6660 ELISA Kit, DPKN ELISA Kit, Dmel\\CG2049 ELISA Kit, Dpkn ELISA Kit, PRK2 ELISA Kit, Pk45C ELISA Kit, Pk?3 ELISA Kit, dPKN ELISA Kit, l(2)06736 ELISA Kit, l(2)45Ca ELISA Kit, v(2)rG232 ELISA Kit, protein kinase N1 ELISA Kit, protein kinase N1b ELISA Kit, Protein kinase N ELISA Kit, PKN1 ELISA Kit, Pkn1 ELISA Kit, pkn1b ELISA Kit, pkn1 ELISA Kit, Pkn ELISA Kit

Background:

Gene Name: Protein Kinase N1

Gene Aliases: PAK1, DBK, PRK1, PRKCL1, Protein Kinase C-Like 1, Protein kinase PKN-alpha, Protein-kinase C-related kinase 1, Serine-threonine protein kinase N

Gene ID: 5585

UniProt: Q16512