PLP1 ELISA Kit

Catalog No. ABIN5658278

Human PLP1 ELISA Kit, Colorimetric assay for quantification of Human PLP1.

Quick Overview for PLP1 ELISA Kit (ABIN5658278)

Target: PLP1 (Proteolipid Protein 1 (PLP1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 3.12 ng/mL - 200 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details PLP1 ELISA Kit

Minimum Detection Limit: 3.12 ng/mL

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Proteolipid Protein 1, Myelin (PLP1). No significant cross-reactivity or interference between Proteolipid Protein 1, Myelin (PLP1) and analogues was observed.

Sensitivity: 1.12 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to Proteolipid Protein 1, Myelin (PLP1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between Horseradish Peroxidase (HRP) labeled Proteolipid Protein 1, Myelin (PLP1) and unlabeled Proteolipid Protein 1, Myelin (PLP1) (Standards or samples) with the pre-coated antibody specific to Proteolipid Protein 1, Myelin (PLP1). After incubation the unbound conjugate is washed off. The amount of bound HRP conjugate is reverse proportional to the concentration of Proteolipid Protein 1, Myelin (PLP1) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Proteolipid Protein 1, Myelin (PLP1) in the sample.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Proteolipid Protein 1, Myelin (PLP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Proteolipid Protein 1, Myelin (PLP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for PLP1

Target: PLP1 (Proteolipid Protein 1 (PLP1))

Alternative Name: Proteolipid Protein 1, Myelin

Background:

Gene Name: Proteolipid Protein 1, Myelin

Gene Aliases: MMPL, PLP/DM20, PMD, SPG2, Pelizaeus-Merzbacher Disease, Spastic Paraplegia 2,Uncomplicated, Myelin proteolipid protein, Lipophilin