CCL18 CLIA Kit

Catalog No. ABIN5658298

The Human CCL18 ELISA Kit (ABIN5658298) is a Chemiluminescent ELISA Kit designed to quantify Human CCL18.

Quick Overview for CCL18 CLIA Kit (ABIN5658298)

Target: CCL18 (Chemokine (C-C Motif) Ligand 18 (Pulmonary and Activation-Regulated) (CCL18))

Reactivity: Human

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 3.12 ng/mL - 200 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details CCL18 CLIA Kit

Minimum Detection Limit: 3.12 ng/mL

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Pulmonary Activation Regulated Chemokine (PARC). No significant cross-reactivity or interference between Pulmonary Activation Regulated Chemokine (PARC) and analogues was observed.

Sensitivity: 0.13 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Pulmonary Activation Regulated Chemokine (PARC). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Pulmonary Activation Regulated Chemokine (PARC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Pulmonary Activation Regulated Chemokine (PARC) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pulmonary Activation Regulated Chemokine (PARC) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pulmonary Activation Regulated Chemokine (PARC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: CCL18 (Chemokine (C-C Motif) Ligand 18 (Pulmonary and Activation-Regulated) (CCL18))

Alternative Name: Pulmonary Activation Regulated Chemokine

Background:

Gene Name: Pulmonary Activation Regulated Chemokine

Gene Aliases: CCL18, AMAC1, SCYA18, DCCK1, MIP-4, CKb7, Alternative Macrophage Activation Associated CC Chemokine 1, Chemokine C-C-Motif Ligand 18, Macrophage inflammatory protein 4

Gene ID: 6362

UniProt: P55774