RAC1 ELISA Kit

Catalog No. ABIN5658358

Product Details RAC1 ELISA Kit

Target: RAC1

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Ras Related C3 Botulinum Toxin Substrate 1 (Rac1). No significant cross-reactivity or interference between Ras Related C3 Botulinum Toxin Substrate 1 (Rac1) and analogues was observed.

Sensitivity: 0.059 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ras Related C3 Botulinum Toxin Substrate 1 (Rac1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ras Related C3 Botulinum Toxin Substrate 1 (Rac1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ras Related C3 Botulinum Toxin Substrate 1 (Rac1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ras Related C3 Botulinum Toxin Substrate 1 (Rac1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ras Related C3 Botulinum Toxin Substrate 1 (Rac1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ras Related C3 Botulinum Toxin Substrate 1 (Rac1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for RAC1

Target: RAC1

Alternative Name: Ras Related C3 Botulinum Toxin Substrate 1 (RAC1 Products)

Synonyms: Rac-1 ELISA Kit, TC-25 ELISA Kit, p21-Rac1 ELISA Kit, AL023026 ELISA Kit, D5Ertd559e ELISA Kit, RAC2 ELISA Kit, CRAC1A ELISA Kit, rac1 ELISA Kit, wu:fd16e02 ELISA Kit, zgc:55823 ELISA Kit, zgc:55917 ELISA Kit, zgc:86934 ELISA Kit, 2248 ELISA Kit, CG2248 ELISA Kit, D-Rac ELISA Kit, D-Rac 1 ELISA Kit, D-Rac1 ELISA Kit, DRac ELISA Kit, DRac1 ELISA Kit, DRacA ELISA Kit, Dm Rac1 ELISA Kit, DmRAC ELISA Kit, DmRAC1 ELISA Kit, Dmel\CG2248 ELISA Kit, Dmrac1 ELISA Kit, Drac ELISA Kit, Drac1 ELISA Kit, Drac1a ELISA Kit, DracA ELISA Kit, GTPase Rac1 ELISA Kit, P40792 ELISA Kit, RAC1 ELISA Kit, Rac ELISA Kit, Rac GTPase ELISA Kit, Rac3 ELISA Kit, RacA ELISA Kit, dRac ELISA Kit, dRac1 ELISA Kit, drac1 ELISA Kit, rac ELISA Kit, Xrac ELISA Kit, mig5 ELISA Kit, p21-rac1 ELISA Kit, tc-25 ELISA Kit, Rac family small GTPase 1 ELISA Kit, RAS-related C3 botulinum substrate 1 ELISA Kit, ras-related C3 botulinum toxin substrate 1 ELISA Kit, ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) ELISA Kit, Rac family small GTPase 1a ELISA Kit, CG2248 gene product from transcript CG2248-RB ELISA Kit, Rac family small GTPase 1 L homeolog ELISA Kit, ras homolog family member G ELISA Kit, RAC1 ELISA Kit, Rac1 ELISA Kit, rac1a ELISA Kit, rac1.L ELISA Kit, RHOG ELISA Kit

Background:

Gene Name: Ras Related C3 Botulinum Toxin Substrate 1

Gene Aliases: TC-25, p21-Rac1, MIG5, Cell migration-inducing gene 5 protein, Ras-like protein TC25, p21-Rac1

Pathways: WNT Signaling, Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Thromboxane A2 Receptor Signaling, VEGF Signaling